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Plasmid stability in a recombinant mammalian cell bioprocess

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dc.contributor.authorByung-Soo Kim-
dc.contributor.authorChoi, Cha Yong-
dc.contributor.authorKim, Byung Gee-
dc.date.accessioned2024-06-13T02:26:01Z-
dc.date.available2024-06-13T02:26:01Z-
dc.date.created2018-06-20-
dc.date.issued1992-05-
dc.identifier.citationBIOTECHNOLOGY LETTERS, Vol.14 No.5, pp.351-356-
dc.identifier.issn0141-5492-
dc.identifier.urihttps://hdl.handle.net/10371/204490-
dc.description.abstractA simple experimental method is devised to determine the fraction of plasmid-harboring cells in a bioprocess employing recombinant mammalian cells. The fraction of plasmid-harboring cells decreased as serum content in the growth medium decreased. The relatively higher increase in the generation time of the plasmid-harboring cell was primarily responsible for this decrease. The mathematical expression obtained for this fraction in terms of the two parameters, i.e. the generation time ratio and the plasmid-loss probability, could represent the experimental data extremely well. The numerical values of these parameters could show the inherent insight of the system. It was found that the data plot against time can draw us to a misleading conclusion of the absence of the effect of serum concentration.-
dc.language영어-
dc.publisherCHAPMAN HALL LTD-
dc.titlePlasmid stability in a recombinant mammalian cell bioprocess-
dc.typeArticle-
dc.identifier.doi10.1007/BF01021246-
dc.citation.journaltitleBIOTECHNOLOGY LETTERS-
dc.identifier.wosidA1992HX12200003-
dc.identifier.scopusid2-s2.0-0026647203-
dc.citation.endpage356-
dc.citation.number5-
dc.citation.startpage351-
dc.citation.volume14-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorByung-Soo Kim-
dc.contributor.affiliatedAuthorChoi, Cha Yong-
dc.contributor.affiliatedAuthorKim, Byung Gee-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusBACTERIAL PLASMIDS-
dc.subject.keywordPlusGENETIC STABILITY-
dc.subject.keywordPlusBACILLUS-SUBTILIS-
dc.subject.keywordPlusCOPY NUMBER-
dc.subject.keywordPlusMAINTENANCE-
dc.subject.keywordPlusINSTABILITY-
dc.subject.keywordPlusDERIVATIVES-
dc.subject.keywordPlusCHEMOSTAT-
dc.subject.keywordPlusKINETICS-
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  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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