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Mutual effect of subcutaneously transplanted human adipose-derived stem cells and pancreatic islets within fibrin gel

Cited 31 time in Web of Science Cited 36 time in Scopus
Authors

Bhang, Suk Ho; Jung, Min Jin; Shin, Jung-Youn; La, Wan-Geun; Hwang, Yong Hwa; Kim, Min Jun; Kim, Byung-Soo; Lee, Dong Yun

Issue Date
2013-10
Publisher
ELSEVIER SCI LTD
Citation
BIOMATERIALS, Vol.34 No.30, pp.7247-7256
Abstract
While subcutaneous tissue has been proposed as a potential site for pancreatic islet transplantation, concern remains that the microvasculature of subcutaneous tissue is too poor to support transplanted islets. In an effort to overcome this limitation, we evaluated whether fibrin gel with human adipose-derived stem cells (hADSCs) and rat pancreatic islets could cure diabetes mellitus when transplanted into the subcutaneous space of diabetic mice. Subcutaneously co-transplanted islets and hADSCs showed normalization of the diabetic recipient's blood glucose levels. The result was enhanced by co-treatment of fibroblast growth factor-2 (FGF2) in the fibrin gel. The hADSCs enhanced islet viability after transplantation by secreting various growth factors that can protect islets from hypoxic damage. Afterward, hADSCs could maintain islet viability by recruiting new microvasculature nearby the transplanted islets via overexpression of vascular endothelial growth factor (VEGF). The hADSCs did not directly differentiate into endothelial cells (no detection of biomarkers of human endothelial cells), but showed evidence of differentiation toward insulin-secreting cells (detection of human insulin). Mice receiving islet transplantation alone did not become normoglycemic. Collectively, co-transplantation of fibrin gel with islets and hADSCs will expand the indications for islet transplant therapy and the potential clinical application of cell-based therapy. (C) 2013 Elsevier Ltd. All rights reserved.
ISSN
0142-9612
URI
https://hdl.handle.net/10371/204506
DOI
https://doi.org/10.1016/j.biomaterials.2013.06.018
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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