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Design and characterization of a maltose binding protein-linked growth factor for matrix engineering
Cited 15 time in
Web of Science
Cited 18 time in Scopus
- Authors
- Issue Date
- 2009-11
- Publisher
- Kluwer Academic Publishers
- Citation
- Biotechnology Letters, Vol.31 No.11, pp.1677-1684
- Abstract
- The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm2 at 100 μg protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces. © Springer Science+Business Media B.V. 2009.
- ISSN
- 0141-5492
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