Publications

Detailed Information

Enhanced nerve growth factor efficiency in neural cell culture by immobilization on the culture substrate

DC Field Value Language
dc.contributor.authorBhang, Suk Ho-
dc.contributor.authorLee, Tae-Jin-
dc.contributor.authorYang, Hee Seok-
dc.contributor.authorLa, Wan-Geun-
dc.contributor.authorHan, Ah Mi-
dc.contributor.authorKwon, Yun Hee Kim-
dc.contributor.authorKim, Byung Soo-
dc.date.accessioned2024-06-14T01:03:12Z-
dc.date.available2024-06-14T01:03:12Z-
dc.date.created2018-06-18-
dc.date.issued2009-05-
dc.identifier.citationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.382 No.2, pp.315-320-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://hdl.handle.net/10371/204529-
dc.description.abstractNerve growth factor (NGF) immobilization on a culture Substrate may dramatically reduce the amount of NGF required for pheochromocytoma (PC12) cell culture. Coverslips on which NGF had been immobilized, or with NGF added to the culture medium daily, were used to culture PC12 cells. We examined the effects of adding 5, 10, or 100 ng of NGF to cultures daily, and compared them to the effects of immobilizing 5, 10, or 100 ng of NGF on culture substrates in a single dose. Cultures with 10 or 5 ng NGF added daily showed dramatically decreased cell viability, mitochondrial metabolic activity, and neuronal differentiation compared to cultures with 100 ng NGF added daily, while also exhibiting increased apoptosis. In contrast, a single dose of 100 ng immobilized NGF yielded results similar to 100 ng NGF added daily (total: 300 ng over 3 days), and 10 or 5 ng immobilized NGF showed far better results than 10 or 5 ng NGF added daily. These results demonstrate that NGF immobilization can dramatically reduce the amount of NGF required in neuronal cell Culture. (C) 2009 Elsevier Inc. All rights reserved.-
dc.language영어-
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE-
dc.titleEnhanced nerve growth factor efficiency in neural cell culture by immobilization on the culture substrate-
dc.typeArticle-
dc.identifier.doi10.1016/j.bbrc.2009.03.016-
dc.citation.journaltitleBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.identifier.wosid000265279300017-
dc.identifier.scopusid2-s2.0-63349094179-
dc.citation.endpage320-
dc.citation.number2-
dc.citation.startpage315-
dc.citation.volume382-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorKim, Byung Soo-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusCONTROLLED-RELEASE-
dc.subject.keywordPlusOUTGROWTH-
dc.subject.keywordPlusPLASMA-
dc.subject.keywordPlusLINE-
dc.subject.keywordAuthorImmobilization-
dc.subject.keywordAuthorNerve growth factor-
dc.subject.keywordAuthorNeuronal differentiation-
dc.subject.keywordAuthorPheochromocytoma cell-
Appears in Collections:
Files in This Item:
There are no files associated with this item.

Related Researcher

  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share