Publications
Detailed Information
Enhanced nerve growth factor efficiency in neural cell culture by immobilization on the culture substrate
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bhang, Suk Ho | - |
dc.contributor.author | Lee, Tae-Jin | - |
dc.contributor.author | Yang, Hee Seok | - |
dc.contributor.author | La, Wan-Geun | - |
dc.contributor.author | Han, Ah Mi | - |
dc.contributor.author | Kwon, Yun Hee Kim | - |
dc.contributor.author | Kim, Byung Soo | - |
dc.date.accessioned | 2024-06-14T01:03:12Z | - |
dc.date.available | 2024-06-14T01:03:12Z | - |
dc.date.created | 2018-06-18 | - |
dc.date.issued | 2009-05 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.382 No.2, pp.315-320 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://hdl.handle.net/10371/204529 | - |
dc.description.abstract | Nerve growth factor (NGF) immobilization on a culture Substrate may dramatically reduce the amount of NGF required for pheochromocytoma (PC12) cell culture. Coverslips on which NGF had been immobilized, or with NGF added to the culture medium daily, were used to culture PC12 cells. We examined the effects of adding 5, 10, or 100 ng of NGF to cultures daily, and compared them to the effects of immobilizing 5, 10, or 100 ng of NGF on culture substrates in a single dose. Cultures with 10 or 5 ng NGF added daily showed dramatically decreased cell viability, mitochondrial metabolic activity, and neuronal differentiation compared to cultures with 100 ng NGF added daily, while also exhibiting increased apoptosis. In contrast, a single dose of 100 ng immobilized NGF yielded results similar to 100 ng NGF added daily (total: 300 ng over 3 days), and 10 or 5 ng immobilized NGF showed far better results than 10 or 5 ng NGF added daily. These results demonstrate that NGF immobilization can dramatically reduce the amount of NGF required in neuronal cell Culture. (C) 2009 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Enhanced nerve growth factor efficiency in neural cell culture by immobilization on the culture substrate | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2009.03.016 | - |
dc.citation.journaltitle | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.wosid | 000265279300017 | - |
dc.identifier.scopusid | 2-s2.0-63349094179 | - |
dc.citation.endpage | 320 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 315 | - |
dc.citation.volume | 382 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Byung Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CONTROLLED-RELEASE | - |
dc.subject.keywordPlus | OUTGROWTH | - |
dc.subject.keywordPlus | PLASMA | - |
dc.subject.keywordPlus | LINE | - |
dc.subject.keywordAuthor | Immobilization | - |
dc.subject.keywordAuthor | Nerve growth factor | - |
dc.subject.keywordAuthor | Neuronal differentiation | - |
dc.subject.keywordAuthor | Pheochromocytoma cell | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.