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In vitro cardiomyogenic differentiation of adipose-derived stromal cells using transforming growth factor-beta 1 : In vitro cardiomyogenic differentiation of adipose-derived stromal cells using transforming growth factor-β1

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dc.contributor.authorGwak, So-Jung-
dc.contributor.authorBhang, Suk H.-
dc.contributor.authorYang, Hee S.-
dc.contributor.authorKim, Sang-Soo-
dc.contributor.authorLee, Dae-Hee-
dc.contributor.authorLee, Soo-Hong-
dc.contributor.authorKim, Byung-Soo-
dc.date.accessioned2024-06-14T01:03:15Z-
dc.date.available2024-06-14T01:03:15Z-
dc.date.created2018-06-18-
dc.date.issued2009-04-
dc.identifier.citationCELL BIOCHEMISTRY AND FUNCTION, Vol.27 No.3, pp.148-154-
dc.identifier.issn0263-6484-
dc.identifier.urihttps://hdl.handle.net/10371/204530-
dc.description.abstractTransplanting stem cells differentiated towards a cardiac lineage can regenerate cardiac muscle tissues to treat myocardial infarction. In this study, we tested the hypothesis that transforming growth factor-beta 1 (TGF-beta 1) induces cardiomyogenic differentiation of adipose-derived stromal cells (ADSCs) in vitro. Rat ADSCs were cultured with TGF-beta 1 (10 ng ml(-1)) for 2 weeks in vitro. ADSCs cultured without TGF-beta 1 served as a control. The mRNA expression of cardiac-specific gene was induced by TGF-beta 1, while the control culture did not show cardiac-specific gene expression. Immunocytochemical analyses showed that a small fraction of ADSCs cultured with TGF-beta 1 for 2 weeks stained positively for cardiac myosin heavy chain (MHC) and alpha-sarcomeric actin. Flow cytometric analyses showed that the proportion of cells expressing cardiac MHC increased with TGF-beta 1. However, no mesenchymal differentiation (e.g., osteogenic and adipogenic differentiation) was detected other than cardiomyogenic differentiation. These results showed that TGF-beta 1 induce ADSC cardiomyogenic differentiation in vitro, which could be useful for myocardial infarction stem cell therapy. Copyright (c) 2009 John Wiley & Sons, Ltd.-
dc.language영어-
dc.publisherWILEY-
dc.titleIn vitro cardiomyogenic differentiation of adipose-derived stromal cells using transforming growth factor-beta 1-
dc.title.alternativeIn vitro cardiomyogenic differentiation of adipose-derived stromal cells using transforming growth factor-β1-
dc.typeArticle-
dc.identifier.doi10.1002/cbf.1547-
dc.citation.journaltitleCELL BIOCHEMISTRY AND FUNCTION-
dc.identifier.wosid000265312500005-
dc.identifier.scopusid2-s2.0-65549153711-
dc.citation.endpage154-
dc.citation.number3-
dc.citation.startpage148-
dc.citation.volume27-
dc.description.isOpenAccessY-
dc.contributor.affiliatedAuthorKim, Byung-Soo-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusMESENCHYMAL STEM-CELLS-
dc.subject.keywordPlusHUMAN BONE-MARROW-
dc.subject.keywordPlusMYOCARDIAL-INFARCTION-
dc.subject.keywordPlusTGF-BETA-
dc.subject.keywordPlusTISSUE-
dc.subject.keywordPlusTRANSPLANTATION-
dc.subject.keywordPlusCARDIOMYOCYTES-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusIMPLANTATION-
dc.subject.keywordPlusGROWTH-
dc.subject.keywordAuthoradult stem cell-
dc.subject.keywordAuthoradipose-derived stromal cells-
dc.subject.keywordAuthorcardiomyogenic differentiation-
dc.subject.keywordAuthortransforming growth factor-beta 1-
dc.subject.keywordAuthorosteogenic differentiation-
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