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In vitro cardiomyogenic differentiation of adipose-derived stromal cells using transforming growth factor-beta 1 : In vitro cardiomyogenic differentiation of adipose-derived stromal cells using transforming growth factor-β1
DC Field | Value | Language |
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dc.contributor.author | Gwak, So-Jung | - |
dc.contributor.author | Bhang, Suk H. | - |
dc.contributor.author | Yang, Hee S. | - |
dc.contributor.author | Kim, Sang-Soo | - |
dc.contributor.author | Lee, Dae-Hee | - |
dc.contributor.author | Lee, Soo-Hong | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.date.accessioned | 2024-06-14T01:03:15Z | - |
dc.date.available | 2024-06-14T01:03:15Z | - |
dc.date.created | 2018-06-18 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.citation | CELL BIOCHEMISTRY AND FUNCTION, Vol.27 No.3, pp.148-154 | - |
dc.identifier.issn | 0263-6484 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204530 | - |
dc.description.abstract | Transplanting stem cells differentiated towards a cardiac lineage can regenerate cardiac muscle tissues to treat myocardial infarction. In this study, we tested the hypothesis that transforming growth factor-beta 1 (TGF-beta 1) induces cardiomyogenic differentiation of adipose-derived stromal cells (ADSCs) in vitro. Rat ADSCs were cultured with TGF-beta 1 (10 ng ml(-1)) for 2 weeks in vitro. ADSCs cultured without TGF-beta 1 served as a control. The mRNA expression of cardiac-specific gene was induced by TGF-beta 1, while the control culture did not show cardiac-specific gene expression. Immunocytochemical analyses showed that a small fraction of ADSCs cultured with TGF-beta 1 for 2 weeks stained positively for cardiac myosin heavy chain (MHC) and alpha-sarcomeric actin. Flow cytometric analyses showed that the proportion of cells expressing cardiac MHC increased with TGF-beta 1. However, no mesenchymal differentiation (e.g., osteogenic and adipogenic differentiation) was detected other than cardiomyogenic differentiation. These results showed that TGF-beta 1 induce ADSC cardiomyogenic differentiation in vitro, which could be useful for myocardial infarction stem cell therapy. Copyright (c) 2009 John Wiley & Sons, Ltd. | - |
dc.language | 영어 | - |
dc.publisher | WILEY | - |
dc.title | In vitro cardiomyogenic differentiation of adipose-derived stromal cells using transforming growth factor-beta 1 | - |
dc.title.alternative | In vitro cardiomyogenic differentiation of adipose-derived stromal cells using transforming growth factor-β1 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/cbf.1547 | - |
dc.citation.journaltitle | CELL BIOCHEMISTRY AND FUNCTION | - |
dc.identifier.wosid | 000265312500005 | - |
dc.identifier.scopusid | 2-s2.0-65549153711 | - |
dc.citation.endpage | 154 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 148 | - |
dc.citation.volume | 27 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | HUMAN BONE-MARROW | - |
dc.subject.keywordPlus | MYOCARDIAL-INFARCTION | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | CARDIOMYOCYTES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | IMPLANTATION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | adult stem cell | - |
dc.subject.keywordAuthor | adipose-derived stromal cells | - |
dc.subject.keywordAuthor | cardiomyogenic differentiation | - |
dc.subject.keywordAuthor | transforming growth factor-beta 1 | - |
dc.subject.keywordAuthor | osteogenic differentiation | - |
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