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세포점착성 PLLA-PAA-RGD 지지체를 이용한 연골세포 증식시 생물반응기의 효과 : Effect of Bioreactor System on Chondrocyte Proliferation using Cell Adhesive PLLA-PAA-RGD Scaffolds

Cited 1 time in Web of Science Cited 5 time in Scopus
Authors

Seok-Yang, Hee; Park, Kwideok; Kim, Jae-Jin; Kim, Byung-Soo; Han, Dong Keun

Issue Date
2008-09
Publisher
KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
Citation
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol.5 No.3, pp.498-505
Abstract
Porous poly(L-lactic acid)(PLLA) scaffolds were prepared and then grafted with acrylic acid(AAC) using in situ direct plasma treatment to obtain hydrophilic PLLA-PAA. The obtained PLLA-PAAc was coupled with Gly-Arg-Asp-Gly(GRGD.) as a ligand peptide to get cell-adhesive PLLA-PAAc-RGD. The prepared AA-grafted and RGD-immobilized PLLA films were confirmed from various surface, analyses. Chondrocyte, isolated front rabbit knee articular cartilages was passaged twice before, cell seeding on the PLLA scaffolds. Once seeded on either PLLA scaffolds, they were placed in a multi-functional bioreactor system and an intermittent hydrodynamic pressure(IHP) was applied in 3 bars, while turned on for 2 min and off for 28 min during 3-weeks Culture. Chondrocyte adhesion increased with time and the order of the adhesion was PLLA control < PLLA-PAAc < PLLA-PAAc-RGD. In addition, the contents of total collagen and glycosaminoglycans(GAG) increased in dynamic Culture system as compared with static one. This suggests that the bioreactor may significantly enhance the adhesion and proliferation of chondrocytes irrespective of kinds of PLLA scaffolds.
ISSN
1738-2696
URI
https://hdl.handle.net/10371/204538
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

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