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In vivo bone formation from human embryonic stem cell-derived osteogenic cells in poly(D,L-lactic-co-glycolic acid)/hydroxyapatite composite scaffolds
DC Field | Value | Language |
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dc.contributor.author | Kim, Sinae | - |
dc.contributor.author | Kim, Sang-Soo | - |
dc.contributor.author | Lee, Soo-Hong | - |
dc.contributor.author | Ahn, Seong Eun | - |
dc.contributor.author | Gwak, So-Jung | - |
dc.contributor.author | Song, Joon-Ho | - |
dc.contributor.author | Kim, Byung-Soo | - |
dc.contributor.author | Chung, Hyung-Min | - |
dc.date.accessioned | 2024-06-14T01:04:02Z | - |
dc.date.available | 2024-06-14T01:04:02Z | - |
dc.date.created | 2018-06-18 | - |
dc.date.issued | 2008-03 | - |
dc.identifier.citation | BIOMATERIALS, Vol.29 No.8, pp.1043-1053 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://hdl.handle.net/10371/204545 | - |
dc.description.abstract | We have previously reported the efficient osteogenic differentiation of human embryonic stem cells (hESCs) by co-culture with primary human bone-derived cells (hPBDs) without the use of exogenous factors. In the present study, we explored whether osteogenic cells derived from hESCs (OC-hESCs) using the previously reported method would be capable of regenerating bone tissue in vivo. A three-dimensional porous poly(D,L-lactic-co-glycolic acid)/hydroxyapatite composite scaffold was used as a cell delivery vehicle. In vivo implantation of OC-hESC-seeded scaffolds showed significant bone formation in the subcutaneous sites of immunodeficient mice at 4 and 8 weeks after implantation (n = 5 for each time point). Meanwhile, implantation of the control no cell-seeded scaffolds or human dermal fibroblast-seeded scaffolds did not show any new bone formation. In addition, the presence of BMP-2 (1 mu g/scaffold) enhanced new bone tissue formation in terms of mineralization and the expression of bone-specific genetic markers. According to FISH analysis, implanted OC-hESCs remained in the regeneration sites, which suggested that the implanted cells participated in the formation of new bone. In conclusion, OC-hESCs successfully regenerated bone tissue upon in vivo implantation, and this regeneration can be further enhanced by the administration of BMP-2. These results suggest the clinical feasibility of OC-hESCs as a good alternative source of cells for bone regeneration. (c) 2007 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | In vivo bone formation from human embryonic stem cell-derived osteogenic cells in poly(D,L-lactic-co-glycolic acid)/hydroxyapatite composite scaffolds | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.biomaterials.2007.11.005 | - |
dc.citation.journaltitle | BIOMATERIALS | - |
dc.identifier.wosid | 000253014400009 | - |
dc.identifier.scopusid | 2-s2.0-37349096421 | - |
dc.citation.endpage | 1053 | - |
dc.citation.number | 8 | - |
dc.citation.startpage | 1043 | - |
dc.citation.volume | 29 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Byung-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | VITRO | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | human embryonic stem cells | - |
dc.subject.keywordAuthor | osteogenic differentiation | - |
dc.subject.keywordAuthor | scaffold | - |
dc.subject.keywordAuthor | bone regeneration | - |
dc.subject.keywordAuthor | BMP-2 | - |
dc.subject.keywordAuthor | tissue engineering | - |
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