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Separation of Phosvitin from Egg Yolk without Using Organic Solvents
Cited 8 time in
Web of Science
Cited 9 time in Scopus
- Authors
- Issue Date
- 2013-11
- Publisher
- 아세아·태평양축산학회
- Citation
- Asian-Australasian Journal of Animal Sciences (AJAS), Vol.26 No.11, pp.1622-1629
- Abstract
- The objective of this study was to develop a new method to separate phosvitin from egg yolk without using organic solvents. Phosvitin was extracted from yolk granules using 10% NaCl or 10% (NH4)(2)SO4 (final concentration) and then treated with heat to precipitate the lipoproteins from the extracted solution. The optimal pH for the phosvitin extraction from yolk granules was determined, and the iron-binding ability of the extracted phosvitin (final product) was tested. Adding 10% (NH4)(2)SO4 disrupted the granules, and the subsequent thermal treatment at 90 degrees C for 1 h precipitated low density and high density lipoproteins, which enabled separation of phosvitin by centrifugation. The phosvitin concentration in the extract was significantly higher when the pH of the solution was adjusted to pH >= 9. The purity and recovery rate of phosvitin at the end of the separation process were approximately 78% and 56%, respectively. The separated phosvitin was confirmed to have ferrous and ferric iron binding ability. The advantages of this new method compared with the traditional methods include no organic solvents and high-priced equipment are needed for the separation. Also, this method is more environment and consumer friendly than that of the traditional methods.
- ISSN
- 1011-2367
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Related Researcher
- College of Agriculture and Life Sciences
- Department of Agricultural Biotechnology
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