S-Space College of Medicine/School of Medicine (의과대학/대학원) Immunology (면역학전공) Journal Papers (저널논문_면역학전공)
Mega-dose Vitamin C modulates T cell functions in Balb/c mice only when administered during T cell activation
- Noh, K.; Lim, H.; Moon, S. K.; Kang, J. S.; Lee, W. J.; Lee, D.; Hwang, Y. I.
- Issue Date
- Immunol Lett. 2005 Apr 15;98(1):63-72.
- Animals; Antioxidants/*pharmacology; Ascorbic Acid/*pharmacology; Cell Proliferation; Cytokines/secretion; Dinitrofluorobenzene/immunology; Drug Hypersensitivity/immunology; Hemocyanin/immunology; Hypersensitivity, Delayed/chemically induced/immunology; Immunoglobulin E/immunology; Immunoglobulin G/immunology; Inflammation/drug therapy; Lymphocyte Activation/*drug effects/immunology; Mice; Mice, Inbred BALB C; T-Lymphocytes/*drug effects/immunology/secretion; Time Factors
- Previously we reported that a mega-dose of Vitamin C enhanced the initial stage of delayed-type hypersensitivity reaction in Balb/c mice. In this study its effects were further evaluated as follows. Mice were administered Vitamin C intraperitoneally at 0.625 mg/day or at 5mg/day for variable days before, during, or after being sensitized with DNFB. T cells were isolated in each group and examined. When stimulated antigen-specifically or non-specifically in vitro, mice showed elevated thymidine uptake and a shift of cytokine secretion profiles toward Th1, i.e., elevated levels IL-2, TNF-alpha, and IFN-gamma, and lowered level of the Th2 cytokine IL-4, only when Vitamin C was administered during sensitization. T cells from those mice administered Vitamin C before sensitization or after challenge showed the same T cell properties as those from PBS-treated mice. Mice were also given 0.625 mg/day of Vitamin C during primary and/or secondary immunizations with KLH and secondary specific antibody titers in sera were measured. The total specific antibody titer was lowered in Vitamin C-treated animals whenever treatments were administered, and this was entirely attributed to decreased levels of IgG1 and IgE antibodies. Based on these results, we suggest that an exogenously administered mega-dose of Vitamin C shifts immunity in Balb/c mouse toward Th1 and that these affects occur only when Vitamin C is administered during T cell activation.
- 0165-2478 (Print)
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