S-Space College of Medicine/School of Medicine (의과대학/대학원) Pharmacology (약리학전공) Journal Papers (저널논문_약리학전공)
Acupuncture inhibits microglial activation and inflammatory events in the MPTP-induced mouse model
- Kang, Jun Mo; Park, Hi Joon; Choi, Yeong Gon; Choe, Il Hwan; Park, Jae Hyun; Kim, Yong Sik; Lim, Sabina
- Issue Date
- Brain Res. 2007 Feb 2;1131(1):211-9. Epub 2006 Dec 14.
- Acupuncture/*methods/trends; Animals; Biological Markers/metabolism; Corpus Striatum/metabolism/physiopathology; Cyclooxygenase 2/metabolism; Cytoprotection/*physiology; Disease Models, Animal; Dopamine/biosynthesis; Encephalitis/chemically induced/prevention & control/*therapy; Gliosis/chemically induced/prevention & control/*therapy; Macrophage-1 Antigen/metabolism; Male; Mice; Mice, Inbred C57BL; Microglia/drug effects/*physiology; Nitric Oxide Synthase Type II/metabolism; Parkinsonian Disorders/physiopathology/prevention & control/*therapy; Substantia Nigra/metabolism/physiopathology; Treatment Outcome; Tyrosine 3-Monooxygenase/metabolism; Up-Regulation/physiology
- Using a mouse model of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD), this study investigated on the neuroprotective effects of acupuncture by examining whether acupuncture contributed to inhibiting microglial activation and inflammatory events. C57BL/6 mice were treated with MPTP (30 mg/kg, i.p.) for 5 consecutive days. Acupuncture was then applied to acupoints Yanglingquan (GB34) and Taichong (LR3) starting 2 h after the first MPTP administration and then at 48 h intervals until the mice were sacrificed for analyses at 1, 3, and 7 days after the last MPTP injection. These experiments demonstrated that acupuncture inhibited the decreased of the tyrosine hydroxylase (TH) immunoreactivity (IR) and generated a neuroprotective effects in the striatum (ST) and the substantia nigra (SN) on days 1, 3, and 7 post-MPTP injections. Acupuncture attenuated the increase of macrophage antigen complex-1 (MAC-1), a marker of microglial activation, at 1 and 3 days and reduced the increases in cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression on days 1, 3, and 7. In MPTP group, striatal dopamine (DA) was measured by 46% at 7 days, whereas DA in the acupuncture group was 78%. On the basis of these results, we suggest that acupuncture could be used as a neuroprotective intervention for the purpose of inhibiting microglial activation and inflammatory events in PD.
- 0006-8993 (Print)
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