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Molecular characterization of cDNA encoding porcine IP-10 and induction of porcine endothelial IP-10 in response to human TNF-alpha

Cited 7 time in Web of Science Cited 5 time in Scopus
Authors
Yang, Jaeseok; Choi, Inho; Kim, Sung Dae; Kim, Eun Sil; Cho, Bumrae; Kim, Jae Young; Ahn, Curie
Issue Date
2007-03-03
Publisher
Elsevier
Citation
Vet Immunol Immunopathol. 2007 May 15;117(1-2):124-8. Epub 2007 Feb 3.
Keywords
Amino Acid SequenceAnimalsBase SequenceChemokine CXCL10Chemokines, CXC/biosynthesis/*genetics/immunologyCloning, MolecularDNA, Complementary/chemistry/*geneticsEndothelial Cells/immunologyGraft Rejection/immunologyHumansMolecular Sequence DataRandom Amplified Polymorphic DNA Technique/veterinarySequence AlignmentSwineSwine, MiniatureTumor Necrosis Factor-alpha/immunology/*pharmacology
Abstract
Donor-derived chemokines may play an important role in xenograft rejection, as well as allograft rejection. We have cloned the cDNA encoding porcine IP-10 (interferon-gamma-inducible protein 10), and evaluated its induction in miniature porcine endothelial cells in response to various human cytokines. The cloned sequence is 764 nucleotides long, and the open reading frame consists of 312 nucleotides. The deduced protein sequence includes a predicted mature protein of approximately 83 residues. The comparison of porcine IP-10, and its human, mouse, rat, goat, and sheep counterparts exhibited high similarity among different mammalian species. The sequences of important regulatory elements such as the interferon-stimulated response element (ISRE), and two NF-kappaB binding elements are conserved in the proximal promoter region of porcine IP-10, like other mammalian IP-10s. Human TNF-alpha induced the expression of IP-10 mRNA in porcine endothelial cells, while both human IFN-gamma and human IL-1beta failed. Together, the data of this study suggest that porcine IP-10 may interact with human leukocytes across the species barrier.
ISSN
0165-2427 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17328963

http://hdl.handle.net/10371/24343
DOI
https://doi.org/10.1016/j.vetimm.2007.01.019
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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