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8-Oxo-7,8-dihydro-2'-deoxyguanosine is not salvaged for DNA synthesis in human leukemic U937 cells
Cited 17 time in
Web of Science
Cited 18 time in Scopus
- Authors
- Issue Date
- 2006-03-23
- Publisher
- Taylor & Francis
- Citation
- Free Radic Res. 2006 May;40(5):461-6.
- Keywords
- Cell Line, Tumor ; DNA/*metabolism ; DNA Replication/physiology ; Guanine/*analogs & derivatives/metabolism ; Humans ; Leukemia ; Oxidative Stress/physiology
- Abstract
- 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG), the most common oxidatively modified nucleoside, is released from oxidized DNA and oxidized nucleotide pool. However, little information is available regarding the metabolic pathway of free 8-oxo-dG. In this study, we generated radiolabeled 8-oxo-dG to track its metabolic fate. We report that 8-oxo-dG is neither phosphorylated to 8-oxo-dGMP nor degraded to the free base, 8-oxo-7,8-dihydroguanine (8-oxo-Gua), indicating that 8-oxo-dG is not a substrate for nucleotide synthesis. This result was confirmed by the finding that no radioactivity was detected in the DNA of U937 cells after incubating the cells with radiolabeled 8-oxo-dG. These observations indicate that 8-oxo-dG produced by oxidative stress is not reutilized for DNA synthesis.
- ISSN
- 1071-5762 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16551572
https://hdl.handle.net/10371/24403
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