S-Space College of Medicine/School of Medicine (의과대학/대학원) Microbiology (미생물학전공) Journal Papers (저널논문_미생물학전공)
Rhapontigenin from Rheum undulatum protects against oxidative-stress-induced cell damage through antioxidant activity
- Zhang, Rui; Kang, Kyoung Ah; Piao, Mei Jing; Lee, Kyoung Hwa; Jang, Hye Suk; Park, Min Jeong; Kim, Bum Joon; Kim, Jin Sook; Kim, Young Sup; Ryu, Shi Yong; Hyun, Jin Won
- Issue Date
- Taylor & Francis
- J Toxicol Environ Health A. 2007 Jul;70(13):1155-66.
- Animals; Antioxidants/*pharmacology; Apoptosis/drug effects; Catalase/metabolism; Cricetinae; Cricetulus; DNA Damage/drug effects; Extracellular Signal-Regulated MAP Kinases/metabolism; Fibroblasts/drug effects/physiology; Gene Expression/drug effects; Hydrogen Peroxide; Lipid Peroxidation; Lung/cytology; Oxidative Stress/*drug effects; Phosphorylation; Plant Extracts/*pharmacology; Reactive Oxygen Species/metabolism; Rheum/*chemistry; Signal Transduction/drug effects; Stilbenes/*pharmacology
- The antioxidant properties of rhapontigenin and rhaponticin isolated from Rheum undulatum were investigated. Rhapontigenin was found to scavenge intracellular reactive oxygen species (ROS), the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and hydrogen peroxide (H2O2). The radical scavenging effect of rhapontigenin was more effective than rhaponticin. Rhapontigenin protected against H2O2-induced membrane lipid peroxidation and cellular DNA damage, which are the main targets of oxidative stress-induced cellular damage. The radical scavenging activity of rhapontigenin protected Chinese hamster lung fibroblast (V79-4) cells exposed to H2O2 by inhibiting apoptosis. Rhapontigenin inhibited cell damage induced by serum starvation and was also found to increase the activity of catalase and its protein expression. Further, rhapontigenin increased phosphorylation of extracellular signal-regulated kinase (ERK) and inhibited the activity of activator protein 1 (AP-1), a redox-sensitive transcription factor. In summary, these results suggest that rhapontigenin protects V79-4 cells against oxidative damage by enhancing the cellular antioxidant activity and modulating cellular signal pathways.
- 1528-7394 (Print)
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