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Angiotensin II inhibits inward rectifier K+ channels in rabbit coronary arterial smooth muscle cells through protein kinase Calpha
Cited 27 time in
Web of Science
Cited 32 time in Scopus
- Authors
- Issue Date
- 2006-01-31
- Publisher
- Elsevier
- Citation
- Biochem Biophys Res Commun. 2006 Mar 17;341(3):728-35. Epub 2006 Jan 19.
- Keywords
- Angiotensin II/*pharmacology ; Animals ; Calcium/chemistry/metabolism ; Cations, Divalent/chemistry ; Cells, Cultured ; Electrophysiology ; Female ; Isoenzymes/antagonists & inhibitors/classification/metabolism ; Male ; Muscle, Smooth, Vascular/*drug effects/*metabolism ; Myocytes, Smooth Muscle/*drug effects/*metabolism ; Patch-Clamp Techniques ; Potassium Channels, Inwardly Rectifying/*metabolism ; Protein Kinase C-alpha/antagonists & inhibitors/*metabolism ; Protein Kinase Inhibitors/pharmacology ; Rabbits ; Receptor, Angiotensin, Type 1/metabolism ; Type C Phospholipases/antagonists & inhibitors/metabolism
- Abstract
- We investigated the effects of the vasoconstrictor angiotensin (Ang) II on the whole cell inward rectifier K(+) (Kir) current enzymatically isolated from small-diameter (<100 microm) coronary arterial smooth muscle cells (CASMCs). Ang II inhibited the Kir current in a dose-dependent manner (half inhibition value: 154 nM). Pretreatment with phospholipase C inhibitor and protein kinase C (PKC) inhibitors prevented the Ang II-induced inhibition of the Kir current. The PKC activator reduced the Kir currents. The inhibitory effect of Ang II was reduced by intracellular and extracellular Ca(2+) free condition and by Go6976, which inhibits Ca(2+)-dependent PKC isoforms alpha and beta. However, the inhibitory effect of Ang II was unaffected by a peptide that selectively inhibits the translocation of the epsilon isoform of PKC. Western blot analysis confirmed that PKCalpha, and not PKCbeta, was expressed in small-diameter CASMCs. The Ang II type 1 (AT(1))-receptor antagonist CV-11974 prevented the Ang II-induced inhibition of the Kir current. From these results, we conclude that Ang II inhibits Kir channels through AT(1) receptors by the activation of PKCalpha.
- ISSN
- 0006-291X (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16442501
https://hdl.handle.net/10371/24778
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