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In vivo monitoring of DNA vaccine gene expression using firefly luciferase as a naked DNA
Cited 5 time in
Web of Science
Cited 8 time in Scopus
- Authors
- Issue Date
- 2006-02-18
- Publisher
- Elsevier
- Citation
- Vaccine. 2006 Apr 12;24(16):3057-62. Epub 2006 Jan 30.
- Keywords
- Animals ; Female ; Genes, Reporter ; Image Processing, Computer-Assisted ; Luciferases, Firefly/*analysis/genetics ; Luminescent Measurements ; Lymph Nodes/immunology ; Mice ; Mice, Inbred BALB C ; Models, Animal ; RNA, Messenger/analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Vaccines, DNA/administration & dosage/*genetics/*immunology ; Gene Expression
- Abstract
- The administration of naked DNA into animals is increasing as a research tool to develop DNA vaccine. To monitor the distribution and duration of gene expression of a DNA vaccine in living organisms, we used the naked DNA encoding firefly luciferase (Fluc) as an imaging reporter gene, and evaluated in vivo bioluminescent images in a murine model. We observed bioluminescence at the injection site and at inguinal lymph node from 10h to 24h post-injection when DNA vaccine encoding Fluc (pcDNA3.1-Fluc) was injected into the bilateral posterior flanks in mice. Fluc gene expressions at injection sites and unilateral posterior flank inguinal lymph node were also confirmed by RT-PCR. However, when pcDNA3.1-Fluc was injected into the mid-dorsum bioluminescent signals were observed at the injection site for up to 14 days post-injection, but no bioluminescent signals were detected in inguinal lymph nodes. Concurrent mRNA expressions of Fluc gene at injection sites but not at inguinal lymph nodes were confirmed by RT-PCR. These findings suggest that optical imaging using Fluc could be useful for monitoring the location, intensity and duration of gene expression of naked DNA vaccines in living animals non-invasively and repetitively.
- ISSN
- 0264-410X (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16483696
https://hdl.handle.net/10371/27567
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