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Histone chaperones regulate histone exchange during transcription
Cited 44 time in
Web of Science
Cited 45 time in Scopus
- Authors
- Issue Date
- 2007-10-05
- Publisher
- Nature Publishing Group
- Citation
- EMBO J. 2007 Oct 31;26(21):4467-74. Epub 2007 Oct 4.
- Keywords
- Cell Cycle Proteins/chemistry ; Fungal Proteins/metabolism ; Gene Deletion ; Gene Silencing ; Histones/chemistry/*metabolism ; Molecular Chaperones/*metabolism ; Mutation ; Nuclear Proteins/metabolism ; Nucleosomes/metabolism ; RNA Polymerase II/metabolism ; Repressor Proteins/metabolism ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/chemistry/metabolism ; Transcription, Genetic
- Abstract
- Transcription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a dual histone expression system. The results showed that the exchange of H3/H4 normally occurs during transcription by the histone chaperones. Both Asf1 and HIR are important for histone deposition but have a different effect on histone exchange. While Asf1 mediated incorporation of external H3/H4 and renewal of pre-existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states.
- ISSN
- 1460-2075 (Electronic)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17914459
https://hdl.handle.net/10371/27835
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