Publications
Detailed Information
An evaluation of the neonatal immune system using a listeria infection model
Cited 15 time in
Web of Science
Cited 15 time in Scopus
- Authors
- Issue Date
- 2007-03-16
- Publisher
- Karger
- Citation
- Neonatology. 2007;92(2):83-90. Epub 2007 Mar 14.
- Keywords
- Animals ; Animals, Newborn/*immunology ; Cytokines/biosynthesis/genetics ; Disease Models, Animal ; Immune System/*physiology ; Listeria Infections/genetics/*immunology/mortality ; Listeria monocytogenes/*physiology ; Mice ; Mice, Inbred C57BL ; RNA, Messenger/metabolism ; Survival Rate ; T-Lymphocytes, Cytotoxic/immunology ; Th1 Cells/*immunology ; Th2 Cells/*immunology
- Abstract
- BACKGROUND: T helper 1 (Th1)/T helper 2 (Th2)-biased cytokine regulation may be another reason that neonates are much more susceptible to infectious disease than are adults. OBJECTIVES: We attempted to determine the ability of neonatal mice to direct the Th1 phenotype against Listeria monocytogenes (LM), because LM, an intracellular Gram-positive bacterium, induces profound cellular immunity by Th1 cells in vivo. METHODS: In order to determine whether neonatal mice evidence strong Th1 activity during LM infection, neonatal mice were compared with adult mice with regard to susceptibility to LM, cytotoxic T lymphocyte activity, and cytokine profiles. Neonatal gene profiles relevant to Th1 and Th2 differentiation during LM infection were also compared between neonatal and adult mice, via real-time PCR and RT-PCR. RESULTS: Neonatal mice were found to be far more susceptible to LM infection than adult mice, due to a lack in the induction of cytotoxic T cell activity, coupled with poor IFN-gamma secretion. Further, LM-infected neonatal mice evidenced much lower levels of expression of Th1-type immune components, including IL-12, IFN-gamma, Delta-4 and T-bet, as compared to those features in adult mice. These results may be due to the comparably lower expressions of mannose-bind lectins and some of toll-like receptors (TLRs) such as TLR-5, -6 and -9, necessary mediators to develop Th1 immune responses. CONCLUSIONS: Neonatal mice may not mount an adequate Th1 type immune response due to a significantly lower expression of Th1-type immune components as compared to adult mice, even when forced into a Th1-prone environment.
- ISSN
- 1661-7819 (Electronic)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17361091
https://hdl.handle.net/10371/28465
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.