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Chloride conductance is required for the protein kinase A and Rac1-dependent phosphorylation of moesin at Thr-558 by KCl in PC12 cells
Cited 9 time in
Web of Science
Cited 9 time in Scopus
- Authors
- Issue Date
- 2005-01-07
- Citation
- J Biol Chem. 2005 Apr 1;280(13):12181-9. Epub 2005 Jan 5.
- Keywords
- Animals ; Chloride Channels/*metabolism ; Chlorides/*metabolism ; Culture Media, Serum-Free/pharmacology ; Cyclic AMP-Dependent Protein Kinases/*metabolism ; Electrophoresis, Polyacrylamide Gel ; Immunoblotting ; Microfilament Proteins/chemistry/*metabolism ; PC12 Cells ; Patch-Clamp Techniques ; Phosphorylation ; Plasmids/metabolism ; Potassium/chemistry ; Potassium Chloride/*metabolism ; Rats ; Signal Transduction ; Threonine/*metabolism ; Time Factors ; Transfection ; rac1 GTP-Binding Protein/*metabolism ; rhoA GTP-Binding Protein/metabolism
- Abstract
- Moesin is a member of the ERM family, a family of cross-linkers between the plasma membrane and the actin cytoskeleton, which are known to be activated by phosphorylation. Previously, we reported the RhoA and Rho kinase-dependent phosphorylation of moesin at Thr-558 in hippocampal neuronal cells by glutamate. Here we studied the induction of moesin phosphorylation by KCl (60 mm) in PC12 cells. Moesin phosphorylation at Thr-558 was increased after 2 min of KCl treatment, peaked at 5 min, and returned to the basal level by 60 min. KCl also activated Rac1, but not RhoA, in PC12 cells, and KCl-induced moesin phosphorylation was suppressed in dominant negative Rac1 (N17 Rac1)-expressed cells. The inhibition of protein kinase A (PKA), known as an upstream kinase of Rac1, abolished Rac1 activation and moesin phosphorylation by KCl. Interestingly, the phosphorylation of moesin by KCl was independent of KCl-induced membrane depolarization and calcium influx but was dependent on KCl-induced chloride conductance. 60 mm KCl induced chloride conductance in PC12 cells, and pretreatment with Cl- channel blocker abolished Rac1 activation and moesin phosphorylation by KCl. These results suggest that the phosphorylation of moesin at Thr-558 in PC12 cells by KCl treatment is PKA- and Rac1-dependent and that KCl-induced chloride conductance is involved in the activation of this signaling system.
- ISSN
- 0021-9258 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15634677
https://hdl.handle.net/10371/29646
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