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Involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity
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- Authors
- Issue Date
- 2006-03-08
- Publisher
- WJG Press
- Citation
- World J Gastroenterol. 2006 Feb 7;12(5):796-9.
- Keywords
- Animals ; Biological Clocks/drug effects/*physiology ; Clonazepam/analogs & derivatives/pharmacology ; Female ; Intestine, Small/cytology/drug effects/*metabolism ; Male ; Mice ; Mice, Inbred BALB C ; Mitochondria/drug effects/metabolism ; Nickel/pharmacology ; Patch-Clamp Techniques ; Sodium-Calcium Exchanger/antagonists & inhibitors/*metabolism ; Thiazepines/pharmacology
- Abstract
- AIM: Interstitial cells of Cajal (ICCs) are the pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We have aimed to investigate the involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity in cultured interstitial cells of Cajal. METHODS: Enzymatic digestions were used to dissociate ICCs from the small intestine of a mouse. The whole-cell patch-clamp configuration was used to record membrane currents (voltage clamp) and potentials (current clamp) from cultured ICCs. RESULTS: Clonazepam and CGP37157 inhibited the pacemaking activity of ICCs in a dose-dependent manner. Clonazepam from 20 to 60 micromol/L and CGP37157 from 10 to 30 micromol/L effectively inhibited Ca2+ efflux from mitochondria in pacemaking activity of ICCs. The IC50s of clonazepam and CGP37157 were 37.1 and 18.2 micromol/L, respectively. The addition of 20 micromol/L NiCl2 to the internal solution caused a "wax and wane" phenomenon of pacemaking activity of ICCs. CONCLUSION: These results suggest that mitochondrial Na+-Ca2+ exchange has an important role in intestinal pacemaking activity.
- ISSN
- 1007-9327 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16521198
https://hdl.handle.net/10371/36239
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