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Indole-3-carbinol enhances ultraviolet B-induced apoptosis by sensitizing human melanoma cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, D-S | - |
dc.contributor.author | Jeong, Y-M | - |
dc.contributor.author | Moon, S-I | - |
dc.contributor.author | Kim, S-Y | - |
dc.contributor.author | Kwon, S-B | - |
dc.contributor.author | Park, E-S | - |
dc.contributor.author | Youn, S-W | - |
dc.contributor.author | Park, K-C | - |
dc.date.accessioned | 2010-01-20T02:28:28Z | - |
dc.date.available | 2010-01-20T02:28:28Z | - |
dc.date.issued | 2006-11-07 | - |
dc.identifier.citation | Cell Mol Life Sci. 2006 Nov;63(22):2661-8. | en |
dc.identifier.issn | 1420-682X (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17086378 | - |
dc.identifier.uri | https://hdl.handle.net/10371/39188 | - |
dc.description.abstract | Indole-3-carbinol (I3C) has been found to act against several types of cancer, while ultraviolet B (UVB) is known to induce the apoptosis of human melanoma cells. Here, we investigated whether I3C can sensitize G361 human melanoma cells to UVB-induced apoptosis. We examined the effects of combined I3C and UVB (I3C/UVB) at various dosages. I3C (200 microM)/UVB (50 mJ/cm(2)) synergistically reduced melanoma cell viability, whereas I3C (200 microM) or UVB (50 mJ/cm(2)), separately, had little effect on cell viability. DNA fragmentation assays indicated that I3C/UVB induced apoptosis. Further results show that I3C/UVB activates caspase-8, -3, and Bid and causes the cleavage of poly(ADP-ribose) polymerase. Moreover, I3C decreased the expression of the anti-apoptotic protein, Bcl-2, whereas UVB increased the translocation of Bax to mitochondria. Thus, an increased Bax/Bcl-2 ratio by I3C/UVB may result in melanoma apoptosis. In conclusion, our study demonstrated that I3C sensitizes human melanoma cells by down-regulating Bcl-2. | en |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | en |
dc.subject | Apoptosis/*drug effects/*radiation effects | en |
dc.subject | BH3 Interacting Domain Death Agonist Protein/metabolism | en |
dc.subject | Caspase 3/metabolism | en |
dc.subject | Caspase 8/metabolism | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Cell Survival/drug effects/radiation effects | en |
dc.subject | Drug Synergism | en |
dc.subject | Humans | en |
dc.subject | Indoles/*pharmacology | en |
dc.subject | Melanoma/*metabolism/*pathology | en |
dc.subject | Poly(ADP-ribose) Polymerases/metabolism | en |
dc.subject | Protein Transport/drug effects | en |
dc.subject | Proto-Oncogene Proteins c-bcl-2/metabolism | en |
dc.subject | bcl-2-Associated X Protein/metabolism | en |
dc.subject | Ultraviolet Rays | - |
dc.title | Indole-3-carbinol enhances ultraviolet B-induced apoptosis by sensitizing human melanoma cells | en |
dc.type | Article | en |
dc.identifier.doi | 10.1007/s00018-006-6306-1 | - |
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