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Indole-3-carbinol enhances ultraviolet B-induced apoptosis by sensitizing human melanoma cells

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dc.contributor.authorKim, D-S-
dc.contributor.authorJeong, Y-M-
dc.contributor.authorMoon, S-I-
dc.contributor.authorKim, S-Y-
dc.contributor.authorKwon, S-B-
dc.contributor.authorPark, E-S-
dc.contributor.authorYoun, S-W-
dc.contributor.authorPark, K-C-
dc.date.accessioned2010-01-20T02:28:28Z-
dc.date.available2010-01-20T02:28:28Z-
dc.date.issued2006-11-07-
dc.identifier.citationCell Mol Life Sci. 2006 Nov;63(22):2661-8.en
dc.identifier.issn1420-682X (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17086378-
dc.identifier.urihttps://hdl.handle.net/10371/39188-
dc.description.abstractIndole-3-carbinol (I3C) has been found to act against several types of cancer, while ultraviolet B (UVB) is known to induce the apoptosis of human melanoma cells. Here, we investigated whether I3C can sensitize G361 human melanoma cells to UVB-induced apoptosis. We examined the effects of combined I3C and UVB (I3C/UVB) at various dosages. I3C (200 microM)/UVB (50 mJ/cm(2)) synergistically reduced melanoma cell viability, whereas I3C (200 microM) or UVB (50 mJ/cm(2)), separately, had little effect on cell viability. DNA fragmentation assays indicated that I3C/UVB induced apoptosis. Further results show that I3C/UVB activates caspase-8, -3, and Bid and causes the cleavage of poly(ADP-ribose) polymerase. Moreover, I3C decreased the expression of the anti-apoptotic protein, Bcl-2, whereas UVB increased the translocation of Bax to mitochondria. Thus, an increased Bax/Bcl-2 ratio by I3C/UVB may result in melanoma apoptosis. In conclusion, our study demonstrated that I3C sensitizes human melanoma cells by down-regulating Bcl-2.en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.subjectApoptosis/*drug effects/*radiation effectsen
dc.subjectBH3 Interacting Domain Death Agonist Protein/metabolismen
dc.subjectCaspase 3/metabolismen
dc.subjectCaspase 8/metabolismen
dc.subjectCell Line, Tumoren
dc.subjectCell Survival/drug effects/radiation effectsen
dc.subjectDrug Synergismen
dc.subjectHumansen
dc.subjectIndoles/*pharmacologyen
dc.subjectMelanoma/*metabolism/*pathologyen
dc.subjectPoly(ADP-ribose) Polymerases/metabolismen
dc.subjectProtein Transport/drug effectsen
dc.subjectProto-Oncogene Proteins c-bcl-2/metabolismen
dc.subject*Ultraviolet Raysen
dc.subjectbcl-2-Associated X Protein/metabolismen
dc.titleIndole-3-carbinol enhances ultraviolet B-induced apoptosis by sensitizing human melanoma cellsen
dc.typeArticleen
dc.identifier.doi10.1007/s00018-006-6306-1-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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