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Indole-3-carbinol enhances ultraviolet B-induced apoptosis by sensitizing human melanoma cells

Cited 40 time in Web of Science Cited 40 time in Scopus
Authors
Kim, D-S; Jeong, Y-M; Moon, S-I; Kim, S-Y; Kwon, S-B; Park, E-S; Youn, S-W; Park, K-C
Issue Date
2006-11-07
Publisher
Springer Verlag
Citation
Cell Mol Life Sci. 2006 Nov;63(22):2661-8.
Keywords
Apoptosis/*drug effects/*radiation effectsBH3 Interacting Domain Death Agonist Protein/metabolismCaspase 3/metabolismCaspase 8/metabolismCell Line, TumorCell Survival/drug effects/radiation effectsDrug SynergismHumansIndoles/*pharmacologyMelanoma/*metabolism/*pathologyPoly(ADP-ribose) Polymerases/metabolismProtein Transport/drug effectsProto-Oncogene Proteins c-bcl-2/metabolism*Ultraviolet Raysbcl-2-Associated X Protein/metabolism
Abstract
Indole-3-carbinol (I3C) has been found to act against several types of cancer, while ultraviolet B (UVB) is known to induce the apoptosis of human melanoma cells. Here, we investigated whether I3C can sensitize G361 human melanoma cells to UVB-induced apoptosis. We examined the effects of combined I3C and UVB (I3C/UVB) at various dosages. I3C (200 microM)/UVB (50 mJ/cm(2)) synergistically reduced melanoma cell viability, whereas I3C (200 microM) or UVB (50 mJ/cm(2)), separately, had little effect on cell viability. DNA fragmentation assays indicated that I3C/UVB induced apoptosis. Further results show that I3C/UVB activates caspase-8, -3, and Bid and causes the cleavage of poly(ADP-ribose) polymerase. Moreover, I3C decreased the expression of the anti-apoptotic protein, Bcl-2, whereas UVB increased the translocation of Bax to mitochondria. Thus, an increased Bax/Bcl-2 ratio by I3C/UVB may result in melanoma apoptosis. In conclusion, our study demonstrated that I3C sensitizes human melanoma cells by down-regulating Bcl-2.
ISSN
1420-682X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17086378

https://hdl.handle.net/10371/39188
DOI
https://doi.org/10.1007/s00018-006-6306-1
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College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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