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Crystal structure of PilF: functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa

Cited 43 time in Web of Science Cited 42 time in Scopus
Authors
Kim, Kyunggon; Oh, Jongkil; Han, Dohyun; Kim, Eunice EunKyeong; Lee, Byungil; Kim, Youngsoo
Issue Date
2006-01-13
Publisher
Elsevier
Citation
Biochem Biophys Res Commun. 2006 Feb 24;340(4):1028-38. Epub 2005 Dec 27.
Keywords
Amino Acid SequenceBinding SitesBiogenesisComputer SimulationCrystallographyDimerizationFimbriae Proteins/*chemistry/metabolism/*ultrastructureFimbriae, Bacterial/*chemistry/physiology/ultrastructureModels, Biological*Models, MolecularMolecular Sequence DataMultiprotein Complexes/chemistry/ultrastructureProtein BindingProtein ConformationPseudomonas aeruginosa/*chemistry/growth & development/ultrastructureStructure-Activity Relationship
Abstract
PilF is a requisite protein involved in the type 4 pilus biogenesis system from the Gram-negative human pathogenic bacteria, Pseudomonas aeruginosa. We determined the PilF structure at a 2.2A resolution; this includes six tandem tetratrico peptide repeat (TPR) units forming right-handed superhelix. PilF structure was similar to the heat shock protein organizing protein, which interacts with the C-terminal peptide of Hsp90 and Hsp70 via a concave Asn ladder in the inner groove of TPR superhelix. After simulated screening, the C-terminal pentapeptides of PilG, PilU, PilY, and PilZ proved to be a likely candidate binding to PilF, which are ones of 25 necessary components involved in the type 4 pilus biogenesis system. We proposed that PilF would be critical as a bridgehead in protein-protein interaction and thereby, PilF may bind a necessary molecule in type 4 pilus biogenesis system such as PilG, PilU, PilY, and PilZ.
ISSN
0006-291X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16403447

http://hdl.handle.net/10371/39195
DOI
https://doi.org/10.1016/j.bbrc.2005.12.108
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College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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