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High-throughput screening of glycan-binding proteins using miniature pig kidney N-glycan-immobilized beads
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Yun-Gon | - |
dc.contributor.author | Shin, Dong-Sik | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.contributor.author | Gil, Geun-Cheol | - |
dc.contributor.author | Park, Chung-Gyu | - |
dc.contributor.author | Mimura, Yusuke | - |
dc.contributor.author | Cooper, David K C | - |
dc.contributor.author | Rudd, Pauline M | - |
dc.contributor.author | Dwek, Raymond A | - |
dc.contributor.author | Lee, Yoon-Sik | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.date.accessioned | 2010-01-27T08:36:19Z | - |
dc.date.available | 2010-01-27T08:36:19Z | - |
dc.date.issued | 2008-03-22 | - |
dc.identifier.citation | Chem Biol. 2008 Mar;15(3):215-23. | en |
dc.identifier.issn | 1074-5521 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18355721 | - |
dc.identifier.uri | http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VRP-4S3PX1T-8-2&_cdi=6240&_user=168665&_orig=search&_coverDate=03%2F21%2F2008&_sk=999849996&view=c&wchp=dGLbVlz-zSkzV&md5=171bc39c8174db3bc82504d61ac4b07d&ie=/sdarticle.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/45519 | - |
dc.description.abstract | Glycan recognition leading to cell-cell interactions, signaling, and immune responses is mediated by various glycan-binding proteins (GBPs) showing highly diverse ligand specificities. We describe here a rapid glycan immobilization technique via 4-hydrazinobenzoic acid (HBA)-functionalized beads and its application to high-throughput screening of miniature pig kidney N-glycan-binding proteins by using a mass-spectrometric approach. Without any derivatization steps, the purified pig kidney N-glycans were directly immobilized on to HBA-functionalized beads and subsequently used to identify GBPs from human serum. This screening method showed remarkable performance for identifying potential GBPs closely involved in pig-to-human xenograft rejection mediated by human serum, including antibodies, cytokines, complement components, siglec, and CD antigens. Thus, these results demonstrate that the GBP screening method was firmly established by one-step immobilization of the N-glycans on to microsphere and highly sensitive mass-spectrometric analysis. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Animals | en |
dc.subject | Benzoic Acids/metabolism | en |
dc.subject | Graft Rejection | en |
dc.subject | Humans | en |
dc.subject | Kidney/*metabolism | en |
dc.subject | Mass Spectrometry | en |
dc.subject | Polysaccharides/*metabolism | en |
dc.subject | Protein Binding | en |
dc.subject | Proteins/*metabolism | en |
dc.subject | Substrate Specificity | en |
dc.subject | Swine | en |
dc.subject | Transplantation, Heterologous/immunology | en |
dc.subject | Microspheres | - |
dc.subject | Swine, Miniature | - |
dc.title | High-throughput screening of glycan-binding proteins using miniature pig kidney N-glycan-immobilized beads | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김윤곤 | - |
dc.contributor.AlternativeAuthor | 심동식 | - |
dc.contributor.AlternativeAuthor | 양영훈 | - |
dc.contributor.AlternativeAuthor | 길근철 | - |
dc.contributor.AlternativeAuthor | 박정규 | - |
dc.contributor.AlternativeAuthor | 이윤식 | - |
dc.contributor.AlternativeAuthor | 김병기 | - |
dc.identifier.doi | 10.1016/j.chembiol.2008.02.009 | - |
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