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Promoter hypomethylation and reactivation of MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines and cancer tissues

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Kim, Kyung-Hee; Choi, Jin-Sung; Kim, Il-Jin; Ku, Ja-Lok; Park, Jae-Gahb

Issue Date
World J Gastroenterol. 2006 Sep 21;12(35):5651-7.
Antigens, Neoplasm/*genetics/metabolismAntimetabolites, Antineoplastic/pharmacologyAzacitidine/analogs & derivatives/pharmacologyCell Line, TumorColorectal Neoplasms/*genetics/metabolism/pathologyDNA, Neoplasm/genetics/metabolismGene Expression Regulation, Neoplastic/geneticsHumansNeoplasm Proteins/*genetics/metabolismPromoter Regions, Genetic/*geneticsRNA, Messenger/genetics/metabolismStomach Neoplasms/genetics/metabolism/pathologyDNA Methylation
AIM: To verify the expression and methylation status of the MAGE-A1 and MAGE-A3 genes in colorectal cancer tissues and cancer cell lines. METHODS: We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A3 genes by RT-PCR analysis and methylation-specific PCR (MS-PCR), as well as sequencing analysis, after sodium bisulfite modification in 32 colorectal cancer cell lines and 87 cancer tissues. RESULTS: Of the 32 cell lines, MAGE-A1 and MAGE-A3 expressions were observed in 59% and 66%, respectively. Subsequent to sodium bisulfite modification and MS-PCR analysis, the promoter hypomethylation of MAGE-A1 and MAGE-A3 was confirmed in both at 81% each. Promoter hypomethylation of MAGE-A1 and MAGE-A3 in colorectal cancer tissues was observed in 43% and 77%, respectively. Hypomethylation of MAGE-A1 and MAGE-A3 genes in corresponding normal tissues were observed in 2% and 6%, respectively. CONCLUSION: The promoter hypomethylation of MAGE genes up-regulates its expression in colorectal carcinomas as well as in gastric cancers and might play a significant role in the development and progression of human colorectal carcinomas.
1007-9327 (Print)
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