S-Space College of Medicine/School of Medicine (의과대학/대학원) Molecular and Clinical Oncology (분자종양의학전공) Journal Papers (저널논문_분자종양의학전공)
Promoter hypomethylation and reactivation of MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines and cancer tissues
- Kim, Kyung-Hee; Choi, Jin-Sung; Kim, Il-Jin; Ku, Ja-Lok; Park, Jae-Gahb
- Issue Date
- World J Gastroenterol. 2006 Sep 21;12(35):5651-7.
- Antigens, Neoplasm/*genetics/metabolism; Antimetabolites, Antineoplastic/pharmacology; Azacitidine/analogs & derivatives/pharmacology; Cell Line, Tumor; Colorectal Neoplasms/*genetics/metabolism/pathology; *DNA Methylation; DNA, Neoplasm/genetics/metabolism; Gene Expression Regulation, Neoplastic/genetics; Humans; Neoplasm Proteins/*genetics/metabolism; Promoter Regions, Genetic/*genetics; RNA, Messenger/genetics/metabolism; Stomach Neoplasms/genetics/metabolism/pathology
- AIM: To verify the expression and methylation status of the MAGE-A1 and MAGE-A3 genes in colorectal cancer tissues and cancer cell lines. METHODS: We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A3 genes by RT-PCR analysis and methylation-specific PCR (MS-PCR), as well as sequencing analysis, after sodium bisulfite modification in 32 colorectal cancer cell lines and 87 cancer tissues. RESULTS: Of the 32 cell lines, MAGE-A1 and MAGE-A3 expressions were observed in 59% and 66%, respectively. Subsequent to sodium bisulfite modification and MS-PCR analysis, the promoter hypomethylation of MAGE-A1 and MAGE-A3 was confirmed in both at 81% each. Promoter hypomethylation of MAGE-A1 and MAGE-A3 in colorectal cancer tissues was observed in 43% and 77%, respectively. Hypomethylation of MAGE-A1 and MAGE-A3 genes in corresponding normal tissues were observed in 2% and 6%, respectively. CONCLUSION: The promoter hypomethylation of MAGE genes up-regulates its expression in colorectal carcinomas as well as in gastric cancers and might play a significant role in the development and progression of human colorectal carcinomas.
- 1007-9327 (Print)