S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 26 No.2 (1985)
A Study on the Estrus Cycle and Superovulation of the Mouse
마우스의 발정주기(發情酒旗) 및 배란유발(排卵誘發)에 관한 실험
- Lim, Yong Taik; Moon, Shin Yong; Lee, Jin Yong; Chang, Yoon Seok
- Issue Date
- Seoul J Med, Vol.26 No.2, pp. 157-262
- Pregnant Mare's Serum Gonadotropin (PMSG) has been used for superovulation
in animal models by similar techniques used in human in vitro fertilization and embryo
transfer (lVF & ET). Intraperitoneal injection of PMSG and human chorionic gonadotropin
(HCG) 48 hours apart will result in ovulation approximately 14 to 19 hours later. The number
of oocytes ovulated is affected by age, strain, dose of gonadotropin, timing of injections, and
This study was undertaken to evaluate the effect of estrus cycle on the retrieval of mouse
oocytes in conjunction with mouse in vitro fertilization as a quality control testing for Seoul IVF
& ET program. Vaginal smears were made by lavage of the vagina with saline solution using
an eyedropper. We observed the cell types on the Papanicolaou stained smear to confirm the
stage of the mouse estrus cycle.
Superovulation was induced with intraperitoneal injection of 5.0 I.U. PMSG followed by 5.0
l.U. HCG 48 hours later. Oocytes retrieval was done 16 hours after the intraperitoneal
injection of HCG.
Oocytes retrieval rates according to the stage of estrus cycle were 14.9±2.6 in proestrus
stage(N=16), 15.8±3.4 in estrus stage(N=24), 15.7±3.8 in metestrus stage(N=16), and
15.1 ±4.6 in diestrus stage(N = 18). These results revealed that the stage of estrus cycle in
mice did not affect the oocyte retrieval rate.