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Label-free target DNA recognition using oligonucleotide-functionalized polypyrrole nanotubes

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dc.contributor.authorKo, Sungrok-
dc.contributor.authorJang, Jyongsik-
dc.date.accessioned2010-03-17T06:12:15Z-
dc.date.available2010-03-17T06:12:15Z-
dc.date.issued2008-
dc.identifier.citationUltramicroscopy 108 (2008) 1328-1333en
dc.identifier.issn0304-3991-
dc.identifier.urihttps://hdl.handle.net/10371/61336-
dc.description.abstractConjugated polymers for oligonucleotide immobilization offer extraordinary potential as transducers for detecting DNA duplex formation, because the electrical, optical, and electrochemical properties are strongly affected by relatively small perturbations. Moreover, carboxylated conducting polymer
supports are an attractive alternative due to their versatile immobilization with DNA, protein, and enzyme using various pendant groups: –SH, –NH2, and –COOH. Therefore, we report the fabrication of carboxylic acid-functionalized polypyrrole nanotubes (CPPy NTs) using oxidant-impregnated template synthesis. The diverse number of carboxylates on the surface of CPPy NTs was applied to binding sites for amino-terminal oligonucleotides. Conductance of single DNA strands (ssDNA) and hybridized DNA helix were readily measured by means of depositing DNA-functionalized nanotubes on gold leads, and indicated high sensitivity (DR/R0 ¼ 1.7) even at low concentration (1 nmol) of target
DNA. In addition, target DNA concentration was distinguished up to a narrow difference of 2nmol. The successful DNA immobilization on polymer nanotubes was confirmed and visualized by the photoluminescence of fluorescein isothiocyanate (FITC)-tagged target DNA using confocal laser scanning microscopy.
en
dc.description.sponsorshipthe Center for Advanced Materials
Processing under the 21C Frontier R&D Programs of the Ministry of Commerce, Industry and Energy (MOCIE), and by the Fundamental R&D Program for Core Technology of Materials of the MOCIE.
en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectCarboxylated polypyrrole nanotubesen
dc.subjectDNA conductanceen
dc.subjectScanning probe microscopeen
dc.subjectSensorsen
dc.titleLabel-free target DNA recognition using oligonucleotide-functionalized polypyrrole nanotubesen
dc.typeArticleen
dc.contributor.AlternativeAuthor고성록-
dc.contributor.AlternativeAuthor장정식-
dc.identifier.doi10.1016/j.ultramic.2008.04.045-
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