S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Disruption of Nfic Causes Dissociation of Odontoblasts by Interfering With the Formation of Intercellular Junctions and Aberrant Odontoblast Differentiation
- Lee, Tae-Yeon; Lee, Dong-Seol; Kim, Hyun-Man; Ko, Jea Seung; Gronostajski, Richard M.; Cho, Moon-Il; Son, Ho-Hyun; Park, Joo-Cheol
- Issue Date
- Histochemical Society
- J Histochem Cytochem 57:469-476
- We reported previously that Nfic-deficient mice exhibit short and abnormal
molar roots and severely deformed incisors. The objective of this study is to address the
mechanisms responsible for these changes using morphological, IHC, and RT-PCR analysis.
Nfic-deficient mice exhibited aberrant odontoblasts and abnormal dentin formation in molar
roots and the labial crown analog of incisors. The most striking changes observed in these
aberrant odontoblasts were the loss of intercellular junctions and the decreased expression
of ZO-1 and occludin. As a result, they became dissociated, had a round shape, and lost their
cellular polarity and arrangement as a sheet of cells. Furthermore, the dissociated odontoblasts
became trapped in dentin-like mineralized tissue, resembling osteodentin in the
overall morphology. These findings suggest that loss of the Nfic gene interferes with the
formation of intercellular junctions that causes aberrant odontoblast differentiation and
abnormal dentin formation. Collectively, these changes in odontoblasts contributed to development
of molars with short and abnormal roots in Nfic-deficient mice.
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