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Distinct sites in E-cadherin regulate different steps in Drosophila tracheal tube fusion.

Cited 35 time in Web of Science Cited 39 time in Scopus
Authors
Lee, Mihye; Lee, Seungbok; Zadeh, Alireza Dehghani; Kolodziej, Peter A.
Issue Date
2003-12
Publisher
Company of Biologists
Citation
Development 130(24), 5989-5999
Keywords
E-cadherinDrosophilaCytoskeleton
Abstract
We have investigated how E-cadherin controls the
elaboration of adherens junction associated cytoskeletal
structures crucial for assembling tubular networks. During
Drosophila development, tracheal branches are joined at
branch tips through lumens that traverse doughnut-shaped
fusion cells. Fusion cells form E-cadherin contacts
associated with a track that contains F-actin, microtubules,
and Shot, a plakin that binds F-actin and microtubules.
Live imaging reveals that fusion occurs as the fusion cell
apical surfaces meet after invaginating along the track.
Initial track assembly requires E-cadherin binding to b-
catenin. Surprisingly, E-cadherin also controls track
maturation via a juxtamembrane site in the cytoplasmic
domain. Fusion cells expressing an E-cadherin mutant in
this site form incomplete tracks that contain F-actin and
Shot, but lack microtubules. These results indicate that Ecadherin
controls track initiation and maturation using
distinct, evolutionarily conserved signals to F-actin and
microtubules, and employs Shot to promote adherens
junction-associated cytoskeletal assembly.
ISSN
0950-1991
Language
English
URI
https://hdl.handle.net/10371/62040
DOI
https://doi.org/10.1242/dev.00806
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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