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Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Khademhosseini, Ali | - |
dc.contributor.author | Suh, Kahp Y. | - |
dc.contributor.author | Yang, Jen M. | - |
dc.contributor.author | Eng, George | - |
dc.contributor.author | Yeh, Judy | - |
dc.contributor.author | Levenberg, Shulamit | - |
dc.contributor.author | Langer, Robert | - |
dc.date.accessioned | 2009-08-06T03:41:12Z | - |
dc.date.available | 2009-08-06T03:41:12Z | - |
dc.date.issued | 2004-08 | - |
dc.identifier.citation | Biomaterials 2004;25:3583-92 | en |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://hdl.handle.net/10371/6222 | - |
dc.description.abstract | Anovel method for patterning cellular co-cultures that uses the layer-by-layer deposition of ionic biopolymers is described. Nonbiofouling
hyaluronic acid (HA) micropatterns were used to immobilize cells and proteins to glass substrates. Subsequent ionic adsorption of poly-l-lysine (PLL) to HApatterns was used to switch the HAsurfaces from cell repulsive to adherent thereby facilitating the adhesion of a second cell type. The utility of this approach to pattern co-cultures of hepatocytes or embryonic stem cells with fibroblasts was demonstrated. In addition, the versatility of this approach to generate patterned co-cultures irrespective of the primary cell seeding and relative adhesion of the seeded cells was demonstrated. Thus, the proposed method may be a useful tool for fabricating controlled cellular co-cultures for cell–cell interaction studies and tissue engineering applications. | en |
dc.description.sponsorship | This research has been supported by NIH (NIH grant # HL60435), NSF (through Bioprocess EngineeringResearch Center) and the U.S. Army (through the Institute of Soldier Nanotechnology). AK is partially funded through a post graduate fellowship (PGS-B)
from Natural Sciences and Engineering Research Council of Canada. Dr. Yang would like to acknowledge the support from the National Science Council of Republic of China under the Grant of NSC-40212-F and the partial support from Chang Gung Hospital. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | patterned co-cultures | en |
dc.subject | Layer-by-layer | en |
dc.subject | Hyaluronic acid | en |
dc.subject | poly-L-lysine | en |
dc.subject | EMBRYONIC STEM-CELLS | en |
dc.subject | DRUG-DELIVERY | en |
dc.subject | HEPATOCYTES | en |
dc.subject | SURFACES | en |
dc.subject | SYSTEMS | en |
dc.subject | PROTEIN | en |
dc.subject | ADHESION | en |
dc.subject | DEXTRAN | en |
dc.subject | CULTURE | en |
dc.subject | FILMS | en |
dc.title | Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 서갑양 | - |
dc.identifier.doi | 10.1016/j.biomaterials.2003.10.033 | - |
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