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Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures

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dc.contributor.authorKhademhosseini, Ali-
dc.contributor.authorSuh, Kahp Y.-
dc.contributor.authorYang, Jen M.-
dc.contributor.authorEng, George-
dc.contributor.authorYeh, Judy-
dc.contributor.authorLevenberg, Shulamit-
dc.contributor.authorLanger, Robert-
dc.date.accessioned2009-08-06T03:41:12Z-
dc.date.available2009-08-06T03:41:12Z-
dc.date.issued2004-08-
dc.identifier.citationBiomaterials 2004;25:3583-92en
dc.identifier.issn0142-9612-
dc.identifier.urihttps://hdl.handle.net/10371/6222-
dc.description.abstractAnovel method for patterning cellular co-cultures that uses the layer-by-layer deposition of ionic biopolymers is described. Nonbiofouling
hyaluronic acid (HA) micropatterns were used to immobilize cells and proteins to glass substrates. Subsequent ionic
adsorption of poly-l-lysine (PLL) to HApatterns was used to switch the HAsurfaces from cell repulsive to adherent thereby
facilitating the adhesion of a second cell type. The utility of this approach to pattern co-cultures of hepatocytes or embryonic stem
cells with fibroblasts was demonstrated. In addition, the versatility of this approach to generate patterned co-cultures irrespective of
the primary cell seeding and relative adhesion of the seeded cells was demonstrated. Thus, the proposed method may be a useful tool
for fabricating controlled cellular co-cultures for cell–cell interaction studies and tissue engineering applications.
en
dc.description.sponsorshipThis research has been supported by NIH (NIH grant # HL60435), NSF (through Bioprocess EngineeringResearch Center) and the U.S. Army (through the Institute of Soldier Nanotechnology). AK is partially funded through a post graduate fellowship (PGS-B)
from Natural Sciences and Engineering Research Council of Canada. Dr. Yang would like to acknowledge the support from the National Science Council of Republic of China under the Grant of NSC-40212-F and the partial support from Chang Gung Hospital.
en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectpatterned co-culturesen
dc.subjectLayer-by-layeren
dc.subjectHyaluronic aciden
dc.subjectpoly-L-lysineen
dc.subjectEMBRYONIC STEM-CELLSen
dc.subjectDRUG-DELIVERYen
dc.subjectHEPATOCYTESen
dc.subjectSURFACESen
dc.subjectSYSTEMSen
dc.subjectPROTEINen
dc.subjectADHESIONen
dc.subjectDEXTRANen
dc.subjectCULTUREen
dc.subjectFILMSen
dc.titleLayer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-culturesen
dc.typeArticleen
dc.contributor.AlternativeAuthor서갑양-
dc.identifier.doi10.1016/j.biomaterials.2003.10.033-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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