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Layer-by-layer deposition of hyaluronic acid and poly-L-lysine for patterned cell co-cultures
Cited 242 time in
Web of Science
Cited 268 time in Scopus
- Authors
- Issue Date
- 2004-08
- Publisher
- Elsevier
- Citation
- Biomaterials 2004;25:3583-92
- Keywords
- patterned co-cultures ; Layer-by-layer ; Hyaluronic acid ; poly-L-lysine ; EMBRYONIC STEM-CELLS ; DRUG-DELIVERY ; HEPATOCYTES ; SURFACES ; SYSTEMS ; PROTEIN ; ADHESION ; DEXTRAN ; CULTURE ; FILMS
- Abstract
- Anovel method for patterning cellular co-cultures that uses the layer-by-layer deposition of ionic biopolymers is described. Nonbiofouling
hyaluronic acid (HA) micropatterns were used to immobilize cells and proteins to glass substrates. Subsequent ionic
adsorption of poly-l-lysine (PLL) to HApatterns was used to switch the HAsurfaces from cell repulsive to adherent thereby
facilitating the adhesion of a second cell type. The utility of this approach to pattern co-cultures of hepatocytes or embryonic stem
cells with fibroblasts was demonstrated. In addition, the versatility of this approach to generate patterned co-cultures irrespective of
the primary cell seeding and relative adhesion of the seeded cells was demonstrated. Thus, the proposed method may be a useful tool
for fabricating controlled cellular co-cultures for cell–cell interaction studies and tissue engineering applications.
- ISSN
- 0142-9612
- Language
- English
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