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A Novel Sensor Platform Based on Aptamer-Conjugated

Cited 99 time in Web of Science Cited 106 time in Scopus
Authors
Yoon, Hyeonseok; June-Hyung, Kim; Nahum, Lee; Byung-Gee, Kim; Jyongsik, Jang
Issue Date
2008
Publisher
Wiley-Blackwell
Citation
ChemBioChem 2008, 9, 634 – 641
Keywords
aptamersbiosensorsfield-effect transistorsnanotubespolypyrrole
Abstract
We first present a simple yet versatile strategy for the functionalization of polymer nanotubes in a controlled fashion. Carboxylicacid- functionalized polypyrrole (CPPy) nanotubes were fabricated by using cylindrical micelle templates in a water-in-oil emulsion system, and the functional carboxyl groups were effectively incorporated
into the polymer backbone during the polymerization by using pyrrole-3-carboxylic acid (P3CA) as a co-monomer without a sophisticated functionalization process. It was noteworthy that the chemical functionality of CPPy nanotubes was readily controlled in both qualitative and quantitative aspects. On the basis of the controlled functionality of CPPy nanotubes, a field-effect transistor (FET) sensor platform was constructed to detect specific
biological entities by using a buffer solution as a liquid-ion gate. The CPPy nanotubes were covalently immobilized onto the microelectrode substrate to make a good electrical contact with the metal electrodes, and thrombin aptamers were bonded to the nanotube surface via covalent linkages as the molecular recognition element. The selective recognition ability of thrombin aptamers combined with the charge transport property of CPPy nanotubes
enabled the direct and label-free electrical detection of thrombin proteins. Upon exposure to thrombin, the CPPy nanotube FET sensors showed a decrease in current flow, which was probably attributed to the dipole–dipole or dipole–charge interaction between thrombin proteins and the aptamer-conjugated
polymer chains. Importantly, the sensor response was tuned by adjusting the chemical functionality of CPPy nanotubes. The efficacy
of CPPy nanotube FET sensors was also demonstrated in human blood serum; this suggests that they may be used for practical diagnosis applications after further optimization.
ISSN
1439-4227
Language
English
URI
https://hdl.handle.net/10371/62261
DOI
https://doi.org/10.1002/cbic.200700660
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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