S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Chitosan sponges as tissue engineering scaffolds for bone formation
- Seol, Yang-Jo; Lee, Jue-Yeon; Park, Yoon-Jeong; Lee, Yong-Moo; Ku, Young; Rhyu, In-Chul; Lee, Seung-Jin; Han, Soo-Boo; Chung, Chong-Pyoung
- Issue Date
- Springer Verlag
- Biotechnol Lett. 26(13):1037-41.
- Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 microm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10(6) (day 1), 11 x 10(6) (day 28) and 12 x 10(6) (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) microg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.
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