Browse

Chitosan sponges as tissue engineering scaffolds for bone formation

Cited 203 time in Web of Science Cited 230 time in Scopus
Authors
Seol, Yang-Jo; Lee, Jue-Yeon; Park, Yoon-Jeong; Lee, Yong-Moo; Ku, Young; Rhyu, In-Chul; Lee, Seung-Jin; Han, Soo-Boo; Chung, Chong-Pyoung
Issue Date
2004-07
Publisher
Springer Verlag
Citation
Biotechnol Lett. 26(13):1037-41.
Keywords
bone formationchitosan spongesosteoblasttissue engineering
Abstract
Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100-200 microm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10(6) (day 1), 11 x 10(6) (day 28) and 12 x 10(6) (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) microg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.
ISSN
0141-5492
Language
English
URI
https://hdl.handle.net/10371/62337
DOI
https://doi.org/10.1023/B:BILE.0000032962.79531.fd
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse