S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Chlorhexidine Gluconate Attenuates the Ability of Lipoteichoic Acid from Enterococcus faecalis to Stimulate Toll-like Receptor 2
- Lee, Jin-Kyung; Baik, Jung Eun; Yun, Cheol-Heui; Lee, Kangseok; Han, Seung Hyun; Lee, WooCheol; Bae, Kwang-Shik; Baek, Seung-Ho; Lee, Yoon; Son, Won-Jun; Kum, Kee-Yeon
- Issue Date
- J Endod 2009;35:212–215
- lipoteichoic acid; Enterococcus faecalis; Chlorhexidine gluconate; tumor necrosis factor-α; intracanal medicament; Toll-like receptor 2
- Chlorhexidine gluconate (CHX) has been widely used as a canal irrigant or an intracanal medicament on account of its antibacterial substantivity. This in vitro study aimed to determine if CHX attenuates the inflammatory activity of Enterococcus faecalis and its major virulence factor, lipoteichoic acid (LTA). An enzyme-linked immunosorbent assay showed that CHX-killed E. faecalis was less potent than heat-killed E. faecalis in the production of tumor necrosis factor α (TNF-α) by a murine macrophage cell line, RAW 264.7 (p < 0.05). Interestingly, pretreatment of LTA with 2% CHX for 6 hours or with 0.2% CHX for 24 hours almost eliminated the TNF-α inducibility (p < 0.05). Furthermore, CHX abrogated the ability of LTA to stimulate Toll-like receptor 2, resulting in the attenuated induction of TNF-α expression. Collectively, our results suggest that CHX can inactivate LTA of E. faecalis leading to the alleviation of inflammatory responses induced by E. faecalis and its LTA.
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