Browse

Molded polyethylene glycol microstructures for capturing cells within microfluidic channels

DC Field Value Language
dc.contributor.authorKhademhosseini, Ali-
dc.contributor.authorYeh, Judy-
dc.contributor.authorJon, Sangyong-
dc.contributor.authorEng, George-
dc.contributor.authorSuh, Kahp Y.-
dc.contributor.authorBurdick, Jason A.-
dc.contributor.authorLanger, Robert-
dc.date.accessioned2009-08-06T06:16:23Z-
dc.date.available2009-08-06T06:16:23Z-
dc.date.issued2004-
dc.identifier.citationLab Chip, 2004, 4, 425-430en
dc.identifier.issn1473-0197-
dc.identifier.urihttps://hdl.handle.net/10371/6272-
dc.description.abstractThe ability to control the deposition and location of adherent and non-adherent cells within microfluidic devices is beneficial for the development of micro-scale bioanalytical tools and high-throughput screening systems. Here, we introduce a simple technique to fabricate poly(ethylene glycol) (PEG) microstructures within microfluidic channels that can be used to dock cells within pre-defined locations. Microstructures of various shapes were used to capture and shear-protect cells despite medium flow in the channel. Using this approach, PEG microwells were fabricated either with exposed or non-exposed substrates. Proteins and cells adhered within microwells with exposed substrates, while non-exposed substrates prevented protein and cell adhesion (although the cells were captured inside the features). Furthermore, immobilized cells remained viable and were stained for cell surface receptors by sequential flow of antibodies and secondary fluorescent probes. With its unique strengths in utility and control, this approach is potentially beneficial for the development of cell-based analytical devices and microreactors that enable the capture and real-time analysis of cells within microchannels, irrespective of cell anchorage properties.en
dc.description.sponsorshipThis research has been supported by the US Army (through the Institute for Soldier Nanotechnology under the Contract DAAD-19-02-D0002 with the US Army Research Office), Draper laboratory and the NSF (through the Bioprocess Engineering Research Center).en
dc.language.isoenen
dc.publisherRoyal Society of Chemistryen
dc.subjectLIVING CELLSen
dc.subjectDEVICESen
dc.subjectCHIPen
dc.subjectFABRICATIONen
dc.subjectCOLIen
dc.titleMolded polyethylene glycol microstructures for capturing cells within microfluidic channelsen
dc.typeArticleen
dc.contributor.AlternativeAuthor서갑양-
dc.identifier.doi10.1039/b404842c-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
Files in This Item:
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse