S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 06 No.1 (1965)
토끼에 있어서 화상, 지혈대 결찰 및 수소이온 농도의 총혈액량과 조직 혈액량에 미치는 영향
Alterations in Total Blood Volume and Tissue Blood Volume Induced by Tourniquet, Burn and Hydrogen Ion Concentration in Rabbits.
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.6 No.1, pp. 21-30
- Alterations in total blood volume, plasma volume (T·
1824), extracellular fluid (NaSCN) and tissue blood volume
(Cr 51--labeled red blood cell) were measured in
50 nembutalized rabbits. Animals were divided into
5 groups: the control group consisted of 14 rabbits. and
in the second (8 rabbits) group after ten minutes of
tourniquet release blood volumes were determined. The
length of tourniquet application on both of hindlimbs
was 30 minutes. In the third group of 12 rabbits burn
was applied on 1/4 of the body surface area by means
of immersing in the boiling water for 20 seconds. In the
fourth group consisting of 10 rabbits acidosis was induced
by means of injecting 0.1 N HCI intravenously. The fifth
group was alkalosis group in which sodium bicarbonate
was injected intravenously. The following results were
1. The normal plasma volume in rabbits was 4.86±
0.264% body weight. Total blood volume (T-1824 and
hematocrit method) was 7.90±0.468%. and extracellular
fluid volume was 25.2±0.839% body weight.
2. After ten minutes of tourniquet release there was
no alteration in plasma and blood volume. Extracellular
fluid volume. however. increased to 29.3%. In the burn
group. plasma volume increased to 6.86% and blood
volume increased to 11.34% accompanied by a slight
tendency of increase in extracellular fluid volume. In
the acidosis and alkalosis group extracellular fluid
volume increased to 31.6%. but plasma and blood
volume remained unaltered.
3. Tissue blood volums expressed pl/gm in the
various tissues of normal rabbits were: brain 11.6.
thyroid gland 116. heart 84.4. liver 95.7. lung 220.
spleen 130. adrenal gland 58.8.
4. In the burn group tissue blood volumes of every
tissue showed an increase.
5. In the tourniquet group heart and adrenal gland
showed increases in the tissue blood volume. In the
other tissues there was no significant alteration in the
tissue blood volume.
6. Tissue blood volume of heart and lung increased
when hydrogen ion concentration was changed. Spleen
tissue blood volume increased in acidosis. Significant
alteration in tissue blood volume was not observed in
other tissues in spite of the change in hydrogen ion