S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 26 No.2 (1985)
A Study on Chemical Mediator of Human Mast Cells - Histamine Release from Human Lung and Tonsillar Mast Cells-
인체 비만세포의 화학 매개체에 관한 연구 -폐 및 편도 비만세포로부터 히스타민 유리
- Kim, You-Young
- Issue Date
- Seoul J Med, Vol.26 No.4, pp. 331-335
- Releasability of histamine, a granule-associated preformed chemical mediator,
was observed from enzymatically dispersed human lung and tonsillar mast cells.
IgE-dependent and calcium ionophore A23187 stimulated histamine release from enzymatically
dispersed human lung mast cells was increased in dose-dependent fashion. Histamine
release reached a maximum in the 1:30 dilution of anti-IgE and at 1.0 ,uM of calcium
ionophore A23187 with human lung mast cells. Above the concentration of secretagogues
which caused maximum histamine release, no further release occurred. Almost identical results
were obtained with enzymatically dispersed human tonsillar mast cells.
Increasing extracellular calcium concentration from 0.05 to 0.5mM caused a concentrationrelated
increase in histamine release from enzymatically dispersed human lung mast cells.