S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Journal Papers (저널논문_수의학과)
Chitosan-graft-polyethylenimine as a gene carrier
- Jiang, Hu-Lin; Kim, You-Kyoung; Arote, Rohidas; Nah, Jae-Woon; Cho, Myung-Haing; Choi, Yun-Jaie; Akaike, Toshihiro; Cho, Chong-Su
- Issue Date
- Journal of Controlled Release, Vol.117 No.2, pp.273-280
- non-viral gene delivery; chitosan; polyethylenimine; chitosan-graft-PEI; cytotoxicity; transfection efficiency; gene therapy
- Chitosans have been proposed as biocompatible alternative cationic polymers that are suitable for non-viral delivery. However, the transfection efficiency of chitosan-DNA nanoparticles is still very low. To improve transfection efficiency, we prepared chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer by an imine reaction between periodate-oxidized chitosan and polyethylenimine (PEI). The molecular weight and composition of the CHI-g-PEI copolymer were characterized, using multi-angle laser scattering (GPC-MALS) and H-1 nuclear magnetic resonance (H-1 NMR), respectively. The copolymer was complexed with plasmid DNA (pDNA) in various copolymer/DNA (N/P) charge ratios, and the complex was characterized. CHI-g-PEI showed good DNA binding ability and high protection of DNA from nuclease attack. Also, with an increase in charge ratio, the sizes of the CHI-g-PEI/DNA complex showed a tendency to decrease, whereas the zeta potential of the complex showed an increase. The CHI-g-PEI copolymer had low cytotoxicity, compared to PEI 25K from cytotoxicity assays. At high N/P ratios, the CHI-g-PEI/DNA complex showed higher transfection efficiency than PEI 25K in HeLa, 293T and HepG2 cell lines. Our results indicate that the CHI-g-PEI copolymer has potential as a gene carrier in vitro. (c) 2006 Elsevier B.V. All rights reserved.
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