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Expression of MAP Kinases and Connexins in the Differentiation of Rat Mammary Epithelial Cells

Cited 7 time in Web of Science Cited 7 time in Scopus
Authors
Seo, Min-Soo; Park, Joon-Suk; Yang, Se-Ran; Park, Ki-Su; Hong, In-Sun; Jo, Eun-Hye; Kang, Kyung-Sun; Lee, Yong Soon
Issue Date
2006
Publisher
Japanese Society of Veterinary Science
Citation
J. Vet. Med. Sci.68: 567-571
Keywords
connexingap junctionMAP kinaserat mammary epithelial cell
Abstract
Gap junctional intercellular communication (GJIC) is involved in the regulation of many cellular processes. MAP kinases are known to affect GJIC and phosphorylation of connexin (Cx). MAP kinases can also be a regulator of cell proliferation and growth. This study was undertaken to show the relevance between expression patterns of Cxs and MAP kinases in rat mammary epithelial cells (RMECs). In order to characterize the RMECs, they were stained with Peanut lectin, which indicates most alveolar epithelial cells, and Thy-1.1 was used as a marker of luminal epithelial cells or myoepithelial cells, respectively. We studied the expression patterns of major gap junction proteins, Cx 26, 32, and 43 in RMECs. Western blot analysis demonstrated that Cx26 gradually decreased from day 2, while Cx32 was expressed constantly from day 1 to 14. Cx43 dramatically increased on day 5 and decreased thereafter. The expression patterns and phosphorylation of ERK1/2 and JNK were similar to Cx43, but expression of p38 was like that of Cx32. These results showed that the MAP kinases that comprise ERK1/2, p38, and JNK were involved in regulation of Cxs. Our data suggests that GJIC plays an important role during rat mammary differentiation and that MAP kinases may be closely related functionally to regulate the gap junction.
ISSN
0916-7250 (print)
1347-7439 (online)
Language
English
URI
http://hdl.handle.net/10371/6491
DOI
https://doi.org/10.1292/jvms.68.567
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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