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Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts
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- Authors
- Issue Date
- 2006-08-31
- Publisher
- Elsevier
- Citation
- Biochem Biophys Res Commun. 349 (2006) 995–1002
- Keywords
- IMUP-1 ; IMUP-2 ; SV40 ; Tumorigenicity ; Fibroblast
- Abstract
- Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently
cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various
cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional
role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/
3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft
agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties,
anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated
in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play
an important role in acquiring a transformed phenotype, tumorigenicity in vivo, and be related to cellular proliferation.
- ISSN
- 0006-291X
http://dx.doi.org/10.1016/j.bbrc.2006.08.137
- Language
- English
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