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A Study on the Property of LDH Isoenzymes of Rabbits

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Authors
Chung, Duk-Jai; Kimm, Sung-Wun
Issue Date
1971-12
Publisher
서울대학교 의과대학
Citation
Seoul J Med, Vol.12 No.4, pp. 209-216
Abstract
The present paper reported on the nature of
pyruvate inhibition of LDH isoenzymes with
and without urea. The crude LDH isoenzymes
were prepared from rabbit heart muscle for
the H-LDH and from rabbit psoas muscle for
the M~LDH, with the following conclusions.
1. It is possible to obtain 4~5 fold purification
of the LDH isoenzymes from rabbit heart
and psoas muscles only with ammonium sulfate
fractionation and DEAE-cellu1eJse treatment.
2. In electrophoresis a new method of staining
is proposed as follows; after electrophoresis
with agarose gel a cellulose acetate strip,
presoaked in the staining mixture, is overlapped
on top of the agarose gel, followed by
incubation and densitometry.
In so doing, a kinetic study on the LDH
isoenzymes could be possible through formazan
staining, instead of UV spectrophotometry.
3. The H-LDH isoenzyme activity is more
markedly inhibited than the M-LDH isoenzyme
by pyruvate.
4. Under the presence of urea, pyruvate
inhibition of H-LDH isoenzyme decreases,
while that of M-LDH isoenzyme increases.
ISSN
0582-6802
Language
English
URI
http://hdl.handle.net/10371/6592
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 12 No.4 (1971)
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