갑상선 호르몬의 변조에 따른 흰쥐 임파기관의 조직화학적 변화

Abstract

To observe the effects of thyroxine on the lymphatic organs, the Sprague-Dawley rats weighing about 200g were classified into 4 groups (Group 1: control, Group 2: thyroidectomy, Group 3: tyroidectomy plus thyroxin treatment in the dose of restoring the metabolism to normal, Group 4: thyroxin treatment in the dose of inducing hyperthyroidism), and from each experimental animals thymus, lymph node and spleen were resected on the Ist, 3rd, 5th and 8th week and fixed in 10% neutral formalin after measuring the organ weight. Each sections were stained with Hematoxylin-Eosin, Methyl Green Pyronin, PAS and Silver stain and observed under the light microscopy. The results are summarized as follows. 1. Weights of the lymphatic organs per body weight decreased after thyroidectomy and increased after thyroxin treatment. 2. After thyroxin treatment: 1) Pyronin positive lymphocytes increased in the corticomedullary junction of thymus, germinal center of the lymph node and periarteriallympho cyte sheath of the spleen. 2) PAS positive cells increased in the corticomedullary junction of thymus. 3) Reticular fibers increased in the medulla of thy mus and perifollicular region of the lymph node. 4) Hematopoietic cells increased in the red pulp of spleen. 3. After thyroidectomy the density of chromatin in the nucleus of thymocytes decreased, and the lymphoid follicles decreased in size and number. 4. The results suggested that thyroxin acts as a mitogen and effects the proliferation and differentiation of lymphocytes.