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Comparative evaluation of nano-fibrous scaffolding for bone regeneration in critical size calvarial defects

Cited 52 time in Web of Science Cited 58 time in Scopus
Authors
Woo, Kyung Mi; Chen, Victor J.; Jung, Hong-Moon; Kim, Tae-Il; Shin, Hong-In; Baek, Jeong-Hwa; Ryoo, Hyun-Mo; Ma, Peter X.
Issue Date
2009-08
Publisher
Mary Ann Liebert
Citation
Tissue Eng Part A. 15, 2155
Abstract
In a previous study we found that nanofibrous poly(l-lactic acid) (PLLA) scaffolds mimicking collagen fibers in
size were superior to solid-walled scaffolds in promoting osteoblast differentiation and bone formation in vitro.
In this study we used an in vivo model to confirm the biological properties of nanofibrous PLLA scaffolds and to
evaluate how effectively they support bone regeneration against solid-walled scaffolds. The scaffolds were
implanted in critical-size defects made on rat calvarial bones. Compared with solid-walled scaffolds, nanofibrous
scaffolds supported substantially more new bone tissue formation, which was confirmed by microcomputed
tomography measurement and von Kossa staining. Goldner’s trichrome staining showed abundant
collagen deposition in nanofibrous scaffolds but not in the control solid-walled scaffolds. The cells in these
scaffolds were immuno-stained strongly for Runx2 and bone sialoprotein (BSP). In contrast, solid-walled scaffolds
implanted in the defects were stained weakly with trichrome, Runx2, and BSP. These in vivo results
demonstrate that nanofibrous architecture enhances osteoblast differentiation and bone formation.
ISSN
1937-335X
Language
English
URI
http://hdl.handle.net/10371/66610
DOI
https://doi.org/10.1089/ten.tea.2008.0433
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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