S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 32 No.2 (1991)
An Experimental Study on the Cytolytic Effects of Natural Killer cells on Various Types of Lung Carcinoma Cell Lines with Augmented HLA Expressions by Gamma Interferon
인체 폐종양 세포주에서 MHC 항원 표현 정도와 자연살세포(NK)의 세포 살해 효과와의 상관관계에 관한 연구
- Lee, Kap No; Park, Seong Hoe; Ham, Eui Keun
- Issue Date
- Seoul J Med, Vol.32 No.2, pp. 57-63
- Lung carcinoma cell lines (NCI-N417, NCI-NI46, NCI-H69, PC-14) were
evaluated on their expression of HLA-class I with W6/32 antibody and HLA-class II
with L243 by flow cytometry. Also examined were the killing and/or cytolytic effects
of allogeneic NK cells and T lymphocytes as effector cells to the lung carcinoma cell
lines by chromium release assay, before and after treatments with gamma interferon.
HLA class I expressions significantly increased after treatment with gamma interferon
(P <0.05), whereas HLA class II expressions showed no differences (P > 0.05). The
killing and/or cytolytic effects of allogeneic NK cells very significantly decreased after
treatment with gamma interferon (P <0.001), while those of the T lymphocytes showed
either no change or a tendancy to decrease (P > 0.05).