S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Theses (Master's Degree_식품·동물생명공학부)
Development of chitosan microsphere for a nasal vaccine delivery system
- Jiang, Hulin
- Issue Date
- 서울대학교 대학원
- 키토산 미립자; Chitosan microspheres; 페길화한 키토산 미립자; Pegylated chitosan microspheres; 백신전달시스템; Vaccine delivery system; 보데텔라 브론키셉티카 데모네트로톡신; Bordetella bronchiseptica Dermonecrotoxin; 종양괴사인자 α; Tumor necrosis factor α; 산화질소; Nitric oxide
- Thesis(master`s)--서울대학교 대학원 :농생명공학부,2004.
- The potential to generate both systemic and local immune response makes the
mucosal system as an attractive site for immunization. However, mucosal
administration of protein and peptide antigens generally results in a poor
immune response. Therefore, successful mucosal vaccination is largely dependent
on the development of effective mucosal adjuvants. In this study, chitosan
microspheres (CMs) and pegylated chitosan microspheres (PCMs) were prepared by
ionic gelation process with tripolyphosphate (TPP). Bordetella Bronchiseptica
Dermonecrotoxin (BBD) as a major virulence factor of a causative agent of
atrophic rhinitis (AR) was loaded to the CMs (or PCMs) for nasal vaccination.
BBD-loaded CMs (or BBD-loaded PCMs) were observed as aggregated shapes although
unloaded CMs (or PCMs) were observed as relatively spherical ones. The average
particle sizes of the BBD-loaded CMs and BBD-loaded PCMs were 4.39 and 5.47 ¥ìm,
respectively. The lower molecular weight of chitosan and the higher medium pH,
the more release of BBD from the BBD-loaded CMs was observed in vitro due to
weaker intermolecular interaction between chitosan and BBD. More BBD was
released from BBD-loaded PCMs than BBD-loaded CMs, and more mechanically
stabilized BBD-loaded PCMs were obtained than BBD-loaded CMs. Tumor necrosis
factor ¥á (TNF-¥á) and nitric oxide (NO) from RAW264.7 cells stimulated with
BBD-loaded CMs (or BBD-loaded PCMs) were gradually secreted, suggesting that BBD
released from BBD-loaded CMs (or BBD-loaded PCMs) has a long-lasting boosting
effect and CMs (or PCMs) are very promising vaccine delivery system.
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