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DNA microarray analysis of Salmonella typhimurium ptsI mutant strain on virulence gene expression
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- Authors
- Advisor
- 유상렬
- Issue Date
- 2004
- Publisher
- 서울대학교 대학원
- Keywords
- Ptsi ; Salmonella ; Spi-2 ; Ompr ; DNA microarray
- Description
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2004.
- Abstract
- In the enteric bacteria, the mutation of ptsI, encodes enzyme I (EI), has been reported to cause
attenuation of virulence in mice. In this study the pathogenic effects of ptsI in Salmonella was
investigated through genomic analysis, using S. typhimurium microarray.
ptsI mutant exhibited the reduced expression of 200 genes and the induced expression of 225
genes more than two folds under the high-oxygen condition, while 196 genes were increased and
136 genes were decreased in the expression under the low-oxygen condition. Most of SPI-2 genes
were reduced in the expression and these might be due to the reduced expression of ompR
regulating SsrA-B. The mutation of ptsI also affected chemotaxis/motility. The expression of
flgLN required for flagellar biosynthesis, motA as a torque generator, and cheY as a chemotaxis
regulator were reduced in the ptsI mutant strain. These results were confirmed in the motility
assay, using semi-solid agar. Taken together, the mutation of ptsI changed the expression of
many genes, such as pckA, pfkA, nuoALMN, cyoABCD, hycCEG, hydN, aceABK and sdhACD required for
the energy production and conversion and the synthesis of vitamin B12
- Language
- English
- URI
- http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000055389
https://hdl.handle.net/10371/67578
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