S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Theses (Master's Degree_식품·동물생명공학부)
Regulation mechanism of HilDC-dependent invF expression in Salmonella pathogenicity island 1
- Issue Date
- 서울대학교 대학원
- Salmonella enteria serovar Typhimurium; Salmonella enteria serovar typhimurium; Invf; HilD; HilC; Spi1; 침투; Invasion
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2004.
- During infection to its host, Salmonella enterica serovar Typhimurium enters the epithelial
cells of the small intestine. This process requires a type III secretion system (TTSS) encoded
on Salmonella pathogenicity islands 1 (SPI-1), a 40kb stretch of DNA located near 63 cs of
chromosome. Four SPI-1 encoded transcriptional regulators, HilD, HilC, HilA and InvF, activate
expression of the SPI-1 TTSS. invF has two promoters. One promoter is activated by HilA and the
other is activated by HilD/C. Effects of regulators and growth conditions on the expression of
invF were examined using primer extension analysis and β-galactosidase assay. HilD/C-dependent
invF expression was activated in exponential phase under high-oxygen condition, and it was
repressed in stationary phase under low-oxygen condition. HilC or HilD overexpression increased
invF expression under both high- and low-oxygen conditions. However, HilD/C dependent invF
expression was influenced more by hilD mutation than by hilC mutation. It is also likely that
invF is autoregulated. HilA-dependent promoter deleted strain was created by λ red
recombination method and invasion assay was performed. In high-oxygen, invasion level was same
with wild type, however, in low-oxygen, invasion of mutant was decreased about half level with
wild type. Additionally in vivo transcription analysis from invF, hilD, and HilD/C-dependent
invF promoter was performed and expressions were increased in invF mutant strain.
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