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Pysicochemical properties and dental caries suppression effect of maltosyl-mannitol
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- Authors
- Advisor
- 문태화
- Issue Date
- 2004
- Publisher
- 서울대학교 대학원
- Keywords
- Maltosyl-mannitol ; Maltosyl-mannitol ; 당전이 ; Transglycosylation ; Bacillus stearothermophilus maltogenic amylase(BSMA) ; Bacillus stearothermophilus maltogenic amylase(bsma) ; 이화학적 특성 ; Physicochemical properties ; 난충치성 ; Non-cariogenicity ; Streptococcus sobrinus NRRL 14555 ; Streptococcus sobrinus nrrl 14555
- Description
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2004.
- Abstract
- The polyols are used as low-calorie bulking agents. They do not undergo Maillard reaction and,
have reduced caramelization and low viscosity. In addition, the polyols generally are toothfriendly,
and suitable for diabetics. Transglycosylation reaction has been used to improve
physicochemical or physiological properties of the existing sugars. Maltogenic amylase from
Bacillus stearothermophilus (BSMA) catalyzes the transglycosylation reaction in the presence of
suitable glycosyl acceptors.
In this study, production of maltosyl-mannitol by a transglycosylation reaction, structural
determination of the transferred product, and determination of some physicochemical properties
and dental caries suppression effect were carried out. Maltosyl-mannitol was produced by the
transglycosylation reaction of BSMA. Maltotriose and mannitol acted as a donor and an acceptor,
respectively. The presence of the mannitol transfer products was confirmed by HPIC and TLC
analyses. The transfer product was purified with Bio-Gel P-2 column chromatography and recycled
preparative high pressure liquid chromatography. The structure was identified by LC-MS, matrixassisted
laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS), and 13Cand
1H-NMR. The transfer product was determined to be 6G-a-D-maltosyl-mannitol, in which the
maltose unit was attatched to the mannitol by an a-(1,6)-glycosidic linkage. The molecular mass
was estimated to be 506.2 and 529.8 daltons from the MALDI-TOF-MS analysis.
- Language
- English
- URI
- http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000055286
https://hdl.handle.net/10371/67587
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