Amplification of VNTR Locus D1S80 in DNA Recovered from Dried Bloodstains

Abstract

As a method of bloodstain testing for the identification of individuals, DIS 80 locus was analyzed in bloodstains using amplified fragment length polymorphism (AMP-FLP) analysis technique. Dried blood samples were prepared on sections of cotton shirt and glass plate with 10-111 aliquots of blood drawn from two individuals, to simulate bloodstain evidence found at crime scenes. After aging at room temperature for 1 month, 3 months and 11 months, DNA was isolated and amplified with primers of DlS80 locus, followed by acrylamide gel electrophoresis and ethidium bromide staining. The amount of DNA recovered from a 10-111 dried blood aliquot was approximately 440 ng, regardless of the dried pattern or the aging. In quality, the DNA became degraded as aging was extended. The AMP-FLP profile of the bloodstain was identical to that of the peripheral blood, which was not affected by the dried pattern or the aging.