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Amplification of VNTR Locus D1S80 in DNA Recovered from Dried Bloodstains
D1S80 locus의 PCR 증폭법을 이용한 혈흔감정

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Authors
Lee, Jung Bin
Issue Date
1991-12
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med, Vol.32 No.4, pp. 217-221
Keywords
PCRVNTRD1S80Bloodstain
Abstract
As a method of bloodstain testing for the identification of individuals, DIS
80 locus was analyzed in bloodstains using amplified fragment length polymorphism
(AMP-FLP) analysis technique. Dried blood samples were prepared on sections of cotton
shirt and glass plate with 10-111 aliquots of blood drawn from two individuals, to simulate
bloodstain evidence found at crime scenes. After aging at room temperature for 1 month,
3 months and 11 months, DNA was isolated and amplified with primers of DlS80
locus, followed by acrylamide gel electrophoresis and ethidium bromide staining. The
amount of DNA recovered from a 10-111 dried blood aliquot was approximately 440
ng, regardless of the dried pattern or the aging. In quality, the DNA became degraded
as aging was extended. The AMP-FLP profile of the bloodstain was identical to that
of the peripheral blood, which was not affected by the dried pattern or the aging.
ISSN
0582-6802
Language
English
URI
http://hdl.handle.net/10371/6759
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 32 No.4 (1991)
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