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Functional analysis of methyl jasmonate using the inducible JMT-overexpressing Arabidopsis
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- Authors
- Advisor
- 최양도
- Issue Date
- 2004
- Publisher
- 서울대학교 대학원
- Keywords
- 주요어: 자스몬산 ; Key words: arabidopsis (arabidopsis thaliana) ; 메틸자스몬산 ; Meja (methyl jasmonate) ; JMT (자스몬산 메틸화 효소) ; Wound ; 형질전환 ; Per-jmt ; 애기장대 (pER-JMT ; Per-pejmt ; pER-PeJMT) ; Active self-regulation ; 특정 발현(local induction) ; Long-distance signal molecule ; 체계적 발현(systemic expression) ; Secondary metabolites. ; 이차 대사산물 ; anthocyanin.
- Description
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2004.
- Abstract
- Methyl jasmonate (MeJA) is a volatile plant cellular regulator that modulates expression of
various genes involved in diverse developmental and defensive processes. A number of its
biosynthetic intermediates, isomers, derivatives and metabolites of this pathway are also known
to be powerful cellular regulator depending on biological system. Though JMT enzyme which is
synthesizing MeJA from JA was cloned in previous study it was hard to distinguish MeJA and JA
in biological roles. To know their real functions, three approaches were attempted in this study.
First method was exogenous MeJA and JA treatment. This experiment shows different pattern of
various genes expression respectively. Second was to control JMT gene expression. Controlling
JMT expression would be one of essential experiment toward resolving the complexity of
jasmonate-mediating plant responses. To obtain more accurate data than constitutive JMTexpressing
palnt inducible-JMT plant, using pER8 plant inducible vector, was generated and used.
Third was using the peroxisomal-targeting JMT expression. JMT enzyme is in cytoplasm, and JA is
synthesized and accumulated in peroxisome. To overcome this limitation of contact, peroxisomaltargeting
JMT (pER-PeJMT) was generated, and tested in JA-response. These three approaches
made us understood that MeJA and JA have the unique and common signal pathways which are
overlapped and separate in some parts, and only MeJA can increase JA-response throughout the
active self-regulation pathway.
Moreover, these result suggest that MeJA should be a long distance signal molecule, and related
to secondary metabolites.
- Language
- English
- URI
- http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000055574
https://hdl.handle.net/10371/67590
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