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Enhancing gene expression through stabilization of mRNA in Escherichia coli

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Authors
박설희
Advisor
유상렬
Issue Date
2004
Publisher
서울대학교 대학원
Keywords
E. colimRNA 안정성Mrnastem-loop structureStabilityhairpinStem-loop structurechaperoneHairpinheat shock proteinChaperoneamylaseHeat shock proteinAmylase
Description
Thesis (master`s)--서울대학교 대학원 :농생명공학부,2004.
Abstract
Expression level of a gene can be modulated by changing stability of mRNA in Escherichia coli.
In order to develop an efficient E. coli expression system, the effects of secondary structures
on the 5-untranslated region (5''-UTR) of mRNA and heat shock proteins on stability of mRNA
were studied. The plasmids conferring different stem-loop structures on the 5''-end of mRNA
(pHY1, pHY2, pHY3, and pHY4) were constructed and the genes encoding amylase were tested. The
degree of stabilization was varied depending on the stem-loop structures. As an additional
stabilizing factor, coexpressed heat shock proteins DnaKJ, GrpE, GroELS encoded by pG-KJE6, or
GroELS encoded by pGro7, were involved in the stabilization of mRNA. The degree of stabilization
was varied depending on the stem-loop structures and variety of heat shock proteins. These
results suggest that secondary structures and heat shock proteins can enhance gene expression by
stabilizing mRNA.
Language
English
URI
http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000055395

https://hdl.handle.net/10371/67591
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Theses (Master's Degree_식품·동물생명공학부)
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